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ATTACHMENT 1 - System Performance Parameters High Output Run Mode*
SBS v4 SPECIFICATIONS
| Read length |
| Dual Flow Cell |
| Single Flow Cell |
| Dual Flow Cell Run Time |
| 1×36 |
| 128-144 Gb |
| 64-72 Gb |
| 29 hrs |
| 2×50 |
| 360-400 Gb |
| 180-200 Gb |
| 2.5 days |
| 2×100 |
| 720-800 Gb |
| 360-400 Gb |
| 5 days |
| 2×125 |
| 900-1 Tb |
| 450-500 Gb |
| 6 days |
| Reads Passing Filter |
| Up to 4 billion single read or 8 billion paired-end reads |
| Up to 2 billion single read or 4 billion paired-end reads |
| Quality |
| Greater than 85% of bases above Q30 at 2×50 bp |
Greater than 80% of bases above Q30 at 2×100 bp Greater than 80% of bases above Q30 at 2×125 bp
SBS v3
| Dual Flow Cell |
| Single Flow Cell |
| Dual Flow Cell Run Time |
| 95-105 Gb |
| 47-52 Gb |
| 2 days |
| 270-300 Gb |
| 135-150 Gb |
| 5.5 days |
| 540-600 Gb |
| 270-300 Gb |
| 11 days |
| Up to 3 billion single read or 6 billion paired-end reads |
| Up to 1.5 billion single read or 3 billion paired-end reads |
Greater than 85% of bases above Q30 at 2×50 bp
* Install specifications comparable to Illumina PhiX control library at supported cluster densities (between 610-678 K clusters/mm2 passing filter using TruSeq v3 Kits or 950-1050 K clusters/mm2 passing filter using HiSeq v4). Run times for high output mode correspond to the sequencing process, only. Performance may vary based on sample quality, cluster density, and other experimental factors.
Rapid Run Mode*
| Read length |
| Dual Flow Cell |
| Single Flow Cell |
| Dual Flow Cell Run Time |
| 1×36 |
| 18-22 Gb |
| 9-11 Gb |
| 7 hr |
| 2×50 |
| 50-60 Gb |
| 25-30 Gb |
| 16 hr |
| 2×100 |
| 100-120 Gb |
| 50-60 Gb |
| 27 hr |
| 2×150 |
| 150-180 Gb |
| 75-90 Gb |
| 40 hr |
| Reads Passing Filter |
| Up to 600 million single read or 1.2 billion paired-end reads |
| Up to 300 million single read or 600 million paired-end reads |
| Quality |
| Greater than 85% of bases above Q30 at 2×50 bp |
Greater than 75% of bases above Q30 at 2×150 bp
*Install specifications comparable to Illumina PhiX control library at supported cluster densities (between 700-820 K clusters/mm2 passing filter using TruSeq Rapid Kits). Run times for rapid run mode correspond to on-board cluster generation (1.5 hr) and sequencing. Performance may vary based on sample quality, cluster density, and other experimental factors.