NIHDA201800138-Purchase_Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: To meet ongoing requirements of NIMH intramural researchers the System Neuroscience Imaging Resource needs to upgrade a Nikon C2 Confocal Microscope System to include a motorized frame, motorized filter wheel, and high capacity stage.

2. Background Information and Objective: Imaging brain sections using either confocal or widefield fluorescence microscopy is an essential step for experiments that are being carried out by many basic science investigators within the NIMH intramural program.

The program recently acquired a Nikon C2 Confocal Microscope System. This is a basic system that lacks features such as motorized optical elements that facilitate sophisticated experiments that investigators would like to perform. The objective of this purchase is to upgrade that system to meet current and future requirements of intramural investigators. Specifically, the goal is to provide a programmable platform on which either widefield or confocal fluorescence microscopy can be efficiently performed and on which these modalities can be efficiently combined in the same specimens, so that rapid widefield scanning over tissue on multiple microscope slides can be used to guide high resolution confocal imaging.

3. Generic Name of Product: Upgrades to existing Nikon C2 Combined

Confocal/Widefield Microscope System.

4. Purchase Description:

I. Microscope frame upgrade--Eclipse Ti2-E with Perfect focus

A) 1 * MEA54000

Ti2-E Inverted Motorized Microscope Stand, Motorized Light Distribution;Eye 100/Left 100/Right 100/Left 80/ Eye Prisms, Sideport FOV 22mm C-Mount or 25mm F-Mount, Manual Switching Intermediate Magnification Lenswith Status Detection 1.0-1.5x (1.5x exchangeable to 2.0x), ManualIn/Out and Manual Focus Bertrand Lens with Status Detection, Built-InHub for Motorized Accessory Connection, Includes 2 ISO C-Mount SideportAdapters, Main Cable, Cable Clips and Toolkit

B) 1 * MEF55037

Ti2-E Controller, Provides Power for Motorized Stand and MotorizedComponents, USB and LAN Connection, Two Connectors Accommodating FourI/O Channels Each

C) 1 * MXA22149

S-TI2-EXT External Trigger Cable

D) 1 * MEB55830

Ti2-T-BS Binocular Body Base Unit (Simple)

E) 1 MEB52520

TC-T-ER Ergo Binocular Tube

F) 2 MAK10110

CFI 10X EYEPIECE F.N. 22MM-NC

G) 1 * MEP59394

Ti2-ND-P Perfect Focus System 4, 340nm-800nm, Six Place DIC MotorizedNosepiece with Motorized Reflector, Measure Unit, Remote Offset FocusMechanism, 45mm IR Block Filter for Diascopic Illumination, Pinch Guard<(>&<)> Drip Hose

H) 1 * MEE59920

Ti2-D-PD Diascopic Illumination Pillar, Tilt-Back Design with LockingMechanism, Centerable Condenser Carrier, Filter Holder for Two Fixed45mm Filter

I) 1 * MEV50010

Ti2-D-SF Diacopic Illumination Slider, Two Fixed 45mm Filter Positionsand Two Sliders for Four 45mm Filters

J) 2 * MBN21816

Filter 45mm, ND16A Multi-Coated Neutral Density

K) 1 * MBN11200

"Filter 45mm, GIF Green Interference (546/60nm)"

L) 1 * MEL51010

Ti2-C-TC-I Intelligent Condenser Turret, Seven Position, ApertureDiaphragm, ND32 Filter In Holder, Labels

M) 1 * MEL56200

T-C-LWD Lens Unit for System Condenser Turret, N.A. 0.52, W.D. 30mm

N) 1 * MEV51030

Ti2-F-FLT-E Motorized Epi Fluorescence Turret, Six Position, Compatiblewith 25mm and 32mm Fluor Cubes

O) 1 * MEE54860

TI2-LA-BF Fixed Main Branch

P) 1 * MEE54730

TI2-LA-FLL Epi Fluorescence Module Large Field of View, Fiber Input

Q) 1 * MEV50020

TI2-F-FSC Circle Field Stop Slider

R) 2 * MXA22147

S-TI2-DCL Daisy Cable Long, Connects Filter Turrets and Manual L-AppsMain Branches to Hub for Intelligent Mirror Position

S) 1 97050 USB 2.0 A-B 15ft Cable

T) 1 79035 "Power Cord 120V, Lead Free "

U) 1 77010603

Adds GA3 capabilities to licenses with exisitng MQS43110 (originalGeneral Analysis GA2):

Adds Image processing and measurement analysisengine for custom and standard routines with support of ND dimensions,3D features, Javascript support, output controls and many datamanagement functions. Available for C, HC,AR or AR Passive Only

V) 1 77060017 DB15HD to 7xBNC cable for TTL control of the SPECTRA X for rapidwavelength switching

W) 1 MEE55800

TI2-DUCF-LHLED Transmitted Light LED Lamp House for use with ConfocalDiascopic Detector, Built-in Fly-Eye Lens for Wide, Even Illumination,45mm Neutral Density ND16 Filter for Bright Field Observation

II. High capacity stage--Prior H139 extended travel stage with linear encoders

X) 1 77011404 PSIII CONTROLLER W/XYZ DRVS, ENCODED

Y) 1 77011545 Joystick and Interactive Control Center (ICC) for X, Y, Z, filterwheels, shutters, Lumen PRO illumination systems and 4th axis devices.For use with Nikon micropscopes.

Z) 1 77011594

ProScan Stage for Nikon Ti Scope; 280x 82mm travel, with linearencoders.

AA) 1 77011652 Nine holder insert for 1 x 3 inch slides for H139 Stage for invertedNikon Microscope.

III. Filters and emission wheel

BB) 1 * 96359 C-FL DAPI SOLA Hard Coat, High Signal-to-Noise, Zero Shift Filter Set,Excitation:

395/25nm (382.5-407.5nm), Emission: 460/50nm (435-485nm),Dichroic Mirror: 425nm

CC) 1 77074658

"DAPI/FITC/CY3/CY5 Emission Filter Set for Spectra X for use with77074657.Emitter:

ET435/26m, 32mmEmitter: ET515/30m, 32mmEmitter: ET595/40m, 32mmEmitter:

ET705/72m, 32mm"

DD) 1 77074660

"CFP/YFP/mCherry Emission Filter Set for Spectra X for use with77074659.Emitter:

ET475/20m, 32mmEmitter: ET540/21m, 32mmEmitter: ET632/60m, 32mm"

EE) 1 * MEE54405

Ti2-P-FWB-E Motorized Barrier Filter Wheel for Sideport, 7 Positionsfor either 25mm or 32mm Filters, C-Mount Included (50ms to adjacentpositions)

5. Salient characteristics: A) Replaces existing frame in Nikon C2 confocal system with motorized Ti2e frame, B) Adds high capacity Prior H139 stage, C) Adds motorized filter wheel with filters for 7 fluorescent ranges, D) Integrates all components with existing hardware and software.

6. All items fully warrantied for 1 year. Equipment is expected to be fully functional for greater than 5 years with minor service, as required. Service after one year may be purchased as an annual contract or on an as needed basis.

7. Quantity: 1

8. Delivery Date: 60-90 days after receipt of order.

9. Option(s): None