New_JA_Streamlined_BELOW_SAT-GeoMx.docx

JUSTIFICATION & APPROVAL

Below the Simplified Acquisition Threshold ($10,000 - $250,000)

Project Title: nanoString GeoMx Digital Spatial Profiler

A. Name and address of the proposed contractor(s) nanoString Technologies, 530 Fairview Avenue N Suite 2000, Seattle, WA 98109 B. Describe why only the requested supply/service will meet the government’s needs In multiple NIAAA labs are interested in spatial transcriptomics approach. The nanostring GeoMx Digital Spatial Profiler is a novel digital technology (proprietary to NanoString) that is based on multiplexed measurement of protein and nucleic acids and offers unparalleled levels of precision coupled with the ability to quantify up to 96 proteins and over 18,000 RNA targets on a formalin-fixed, paraffin-embedded (FFPE) or a fresh frozen (FF) tissue section on a microscope slide. This technology will allow us to expand our technical capabilities to conduct spatial biology, which will uncover critical pathologic processed, eventually may help to identify therapeutic targets in multiple disorders.Using this technology will allow us to analyze detailed pathologic pathways analysis in as shorter time, with minimum sample requirement and cost-effective manner. Currently we have already nanostring nCounter Max analyzer, which is the digital anaylzer of the GeoMx Digital Spatial profiler. Therefore, the new aquation will also work in synchrony with the existing system. Besides, combined of these system could detect fragmented nucleic acid samples from formalin fixed parafilm embedded histological tissue samples. This could give flexibility to use with human archive samples to make clinical relevance of our preclinical findings.

C. Describe why only the proposed contractor is capable of providing the requested supply/service

The nCounter GeoMx Digital Spatial Profiler and associated reagents required to operate the system are only available from NanoString Technologies, Inc. Therefore, NanoString Technologies, Inc is the sole source for the requested nCounter GeoMx DSP system.