JA_Simplified_All-in-one_Fluorescence_Microscope.docx

JUSTIFICATION & APPROVAL

Below the Simplified Acquisition Threshold ($10,000 - $250,000)

Project Title: All-in-one Fluorescence Microscope

A. Name and address of the proposed contractor(s)

KEYENCE CORPORATION OF AMERICA

500 Park Boulevard Suite 200 Itasca, IL 60143

PHONE: 888-539-3623

B. Describe why only the requested supply/service will meet the government’s needs

LNCB at NIAAA performs fluorescence imaging experiments of whole brain tissues on multiple slides. This All-in-one Fluorescence Miscroscope by Keyence will (1) provide simple setup and easy operation with excellent imaging quality, (2) save lab space since it comes with a built-in darkroom, and (3) enable us to perform a batch acquisition and analysis of large data sets from multiple slides, thereby saving time.

C. Describe why only the proposed contractor is capable of providing the requested supply/service

This automated high-resolution microscope with specimen enclosure is unique in design and performance, and not available from any other vendors. The company has been around and provided a number of products with cutting-edge technology. It was recommended by our collaborators and was impressive when the company representative demonstrated the system on site.