Q A_Amendment_1.docx

Solicitation HU0001-15-Q-D036 Q&A Amendment #1 Q1) In the desired specification section, what is mean by “State of the art methodology for dye and probe based PCR quantification analysis?

A1) “ State of the art methodology for dye and probe based PCR quantification analysis” means that we want a digital PCR system , which can determine absolute quantification value s for DNA molecules in assayed fluids without the need of standard curve or calibration of reference sample. The methodology is different from the old fashion- PCR; technically it is advanced system for next generation sequencing, single cell mRNA analysis, pathogen detection etc.

Q2) Is your requestor looking for pricing for an automated system, or a manual Digital system?

A2) We are looking for an Automated running system, especially droplet digital PCR, which allows for absolute quantification of specific DNA molecule with a numerical count of copies per volume.

However, for sample preparation, it’s manual.

Q3) In the desired specification section it states: “Ability to assay from SYBR-based reactions”. EvaGreen is a third generation dsDNA binding dye which offers several advantages over SYBR Green,” “It is less inhibitory to PCR and can be used under saturating conditions to generate greater fluorescent signals. EvaGreen is also well suited for HRM [high resolution melt] analysis.”

A3) EvaGreen will meet the desired specification. The amended RFQ adding EvaGreen is attached.