B. AP PD.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose:

The National Center for Advancing Translational Sciences (NCATS) Division of Preclinical Innovation (DPI) Stem Cell Translation Laboratory (SCTL) requires a Cellaca PLX Image Cytometer System.

2. Background Information and Objective:

To establish and move stem cell technologies forward through a more centralized effort, NIH has launched the Stem Cell Translation Laboratory (SCTL) within NCATS. Part of the NIH Common Fund’s, the goal of the SCTL is to bring iPSC technology closer to clinical application, drug discovery and regenerative medicine. Through SCTL, NCATS will provide researchers across various disciplines and organizations with the ability to establish collaborations to advance the translation of regenerative medicine applications.

Through a multidisciplinary collaborative team approach, NCATS’ SCTL scientists aim to:

• Establish detailed quality control (QC) standards to define pluripotency and differentiated cell types.

• Develop methods to assess variations in cultured cells derived from iPSCs.

• Develop standard methods to produce mature cells meeting the QC standards above.

• Discover, validate and disseminate small molecule reagents to replace expensive recombinant proteins, xenogenic material and undefined media components in cell differentiation protocols.

This instrument will be used upon routine iPSC maintenance and differentiation culture to measure cell viability and immunophenotypes. This is a required instrument in cell culture labs as it provides precise information about the health, number of cells, and immunophenotypes of the cells being cultured by a biologist, while removing inter-operator variability that happens when counting manually. This equipment will be used routinely for internal and external collaborations. It will help us to perform the experiments in a steady and fast-paced manner.

3. Generic Name of Product: Image cytometry system

4. Purchase Description:

- Cellaca PLX Image Cytometer with bright f ield & fluorescence imaging

5. Salient characteristics

Cellaca PLX Image Cytometer is a stand-alone system for high-throughput cell counting with brightfield and five fluorescent channels for highly accurate multi-channel analysis.

Channels: Brightfield, Blue, Green Orange, Red, Far Red, Number of Fluorescent Channels : 13 (6 per scan) Excitation LED: 370m 4765, 531, 628nm Emission filter: 452, 534, 605, 655,692 nm Commonly used compatible dyes and assays: Trypan Blue, AO/PI, Hoechst, DAPI, GFP, RFP, CMFDA, Calcein AM, 7AAD, Annexin V, PE, APC, KIRAVIA Blue 520TM* Counting Speed per wel : Fluorescence 4-channel scan – Less than 1 minute Volume (per sample): 15 ul in slide, 50-200ul in counting plates Accessories:

Computer controller One subscription license to FCS Express - Flow Cytometry software (for 2 years) USB cable Power supply High Speed Imaging Cytometer Computer System (with Microsoft Windows 10, DeNovo Software FCS Express 7)

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

Proprietary image acquisition and analysis software USB and power cables 12-month warranty

Quantity: 1 system

6. Delivery Date:

30 Days after receipt of order from NCATS

7. Period of Performance:

One time shipment

9. Option(s): N/A