Attch 1 - Purchase Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION- Tunable Laser System for Multiphoton Microscopes

1. Statement of Need and Purpose:

The National Institute of Neurological Disorders and Stroke (NINDS) is establishing a new laboratory: The Spatial Navigation and Memory Unit (SNMU). The laboratory will use cutting-edge imaging and behavioral approaches to investigate the neural basis of spatial navigation and memory at the circuit, synaptic, molecular and cellular levels. A tunable laser system will be necessary to perform the imaging experiments.

2. Background Information and Objective:

The SNMU aims to investigate the neural basis of spatial navigation and memory.

Specifically, the experiments in the laboratory will largely use an in vivo two-photon imaging approach to measure calcium dynamics of many neurons, observe their structural and molecular properties at the subcellular level, and modulate the activity of individual neurons. The laboratory will include two multiphoton microscopes, which will be capable of performing the above experiments and will require a high power, widely tunable and dual-output laser system. The data collected from this system, including the microscopes and laser, will be used to understand the functional mechanism of the medial entorhinal cortex in spatial navigation.

3. Generic Name of Product:

Tunable Laser System for multiphoton microscope

4. Salient characteristics The NINDS SNMU requires one high power, broadly tunable and dual-output laser (tunable beam and fixed wavelength beam), which must have the following features, the combination of which cannot be provided by other lasers:

System

• Advance Replacement service strategy to minimize user down time, supported by a remote service facility.

• Laser head footprint <0.4m2 to optimize optical table space.

• HALT designed, HASS tested for highest reliability and long lifetime

• Upgradable platform to add fully integrated power modulation of both outputs via fast

AOM control

Tunable beam

• Tuning range of 660nm to 1320nm

• Average power > 2 W at 700nm, >3 W at 800nm, > 2.7 W at 900nm, >2 W at

1000nm, >1.7 W at 1200nm, > 1.4 W at 1300nm

• Pulse duration 100fs at 900nm (sech2 pulse shape)

• Automated dispersion compensation on the tunable output of 0-50000fs2 at 680nm, 0-27000fs2 at 800nm, 0-16000fs2 at 950 nm, 0-12000s2 at 1050nm, and 0-10000fs2 at 1300nm (all pre-compensation values are negative). It must be possible to access the shortest possible pulses out of the box.

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

Fixed Wavelength Beam

• Average power >3.5W at 1040nm

• Pulse duration 140fs (sech2 pulse shape)

5. Quantity:

One system

6. Delivery Date:

The contractor shall deliver and install the equipment within 45 days after receipt of order. Delivery must be FOB destination. On-site training must take place within two (2) weeks of equipment installation. The equipment shall be delivered and installed between the hours of 8:00 am and 5:00 pm, Bethesda, MD local prevailing time. The contractor shall provide an Original Equipment Manufacturer (OEM) certified service technician to perform setup, installation, and an on-site one-day training session for ten staff members.

7. Period of Performance:

N/A

8. Option(s):

N/A