Attachment 2 -Product Description -20-003930.pdf

PURCHASE DESCRIPTION

Yttereium (Yb) Pump Laser

1. Statement of Need and Purpose:

The Section on Critical Brain Dynamics, National Institute of Mental Health (NIMH) requires a pulsed laser for their 2-photon imaging needs to cover a broad range of wavelengths (~600 nm to ~1300 nm). The availability of a broad wavelength range for imaging will allow to combine their imaging technology with new laser stimulation technology (optogenetics) to make the best use of recently developed modern fluorescent probes for neuroscience research.

2. Background Information and Objective:

The Section on Critical Brain Dynamics, NIMH requires a pulsed laser for their 2-photon imaging needs. The Plenz lab studies normal and pathological brain dynamics in awake behaving transgenic mice using laser technology. In short, the imaging laser excites fluorescent molecules that are being expressed in nerve cells of a mouse. This excitation allows for monitoring simultaneously the activity of thousands of neurons in the behaving animal. Using animal models for mental disease, the Plenz lab derives statistical features of normal and pathological brain function in order to identify mechanisms of mental diseases.

The pulse laser system is required due to recent advances in laser technology and fluorescent molecule design. A modern laser for 2-photon imaging needs to cover a broad range of wavelengths to take advantage of modern fluorescence molecules/probes. Specifically, the lab needs to be able to excite probes in the short wavelength range of ~800 nm as well as long wavelength range of up to 1300 nm.

One of the current lasers used in the Plenz lab was purchased approximately 10 years ago and only covers the short wavelength range. The new laser in addition will cover the long wavelength range. By being able to cover a broad wavelength range for imaging, the Plenz lab will be able to combine 2-photon imaging with the most efficient fluorescent probes available to date providing needed flexibility in experimental designs for their experiments and their optical stimulation techniques.

3. Generic Name of Product:

Yttereium (Yb) Pump Laser

4. Purchase Description: Brand Name or Equal

1) Coherent Laser Head: Monaco 1035-80-60: Series – Model Number: 132484

2) Coherent Opera-F: Yb pumped OPA (650-900 nm: 1200-2500nm) with the signal compressor and the idler compressor – Model Number: 1309788

5. Salient characteristics

Technical requirements for acquisition of this system includes the following:

1) Coherent Laser Head: Monaco 1035-80-60: Series - Model Number: 132484

1. Fundamental Center Wavelength (nm) 1035 +/- 5.

2. Output Power (W) 60

3. Energy (uJ) 80 (at 750kHz)

4. Repetition Rate:

Single-shot to 1 MHz Higher rep. rates without AOM pulsepicking:1 to 50 MHz standard

5. Pulsewidth (fs) <350 (typical <280 )

6. Tuning Range <350 fs to >10 ps

7. Spatial Mode TEM00, M2 <1.2

8. Beam Divergence (mrad) <1

9. Beam Diameter at Output (mm, 1/e2) 2.7 ±0.3

10. Beam Circularity (%) > 85

11. Polarization Ratio > 100:1

12. Polarization Direction Vertical ±3°

13. Beam Pointing Stability (µrad/°C) <25

14. Pulse Energy Stability (%) (RMS) <1.5

15. Power Stability (%) (RMS, 2sig) <1.5

16. Warm-up Time (minutes)

Cold Start <45 Warm Start <15

17. Long-term Pointing Stability (µrad) ±25 over 8 hours

18. Head Weight 50 kg (110 lbs.)

19. External Comms RS-232, Ethernet, USB

20. Power Consumption (typical) 48VDC, <500W

2) Coherent Opera-F: Yb pumped OPA (650-900 nm: 1200-2500nm) with the signal compressor and the idler compressor – Model Number: 1309788

1. Opera-F is an Optical Parametric amplifier (OPA) used to extend the turning range of the Coherent Monaco Yb amplifier system. Tt can extend the tuning capability of the Monaco from 650 - 900 nm (signal) and 1200 - 2500 nm (idler).

2. Opera-F system combines the short pulse generation delivered from a non-collinear OPA with the broad turning range and ease of use of a collinear OPA. Opera-F incorporates a white-light seeded non-collinear pre-amplifier followed by a collinear power amplifier the highest levels of performance.

3. Computer-controlled tuning

4. Standard tuning range 650 - 900 nm (signal) and 1200 - 2500 nm (idler).

5. >10% conversion efficiency from pump (signal + idler)

6. Optional compressors to deliver 25-75 fs pulses (signal) and 40-100 fs pulses (idler)

7. Compatible with Monaco amplifier

8. Standard warranty – 13 months from date of shipment or 12 months after installation, whichever comes first

6. Quantity: 1

7. Delivery Date:

The contractor shall deliver and install the equipment within 30-40 calendar days after receipt of order. The contractor shall provide certified and factory trained technicians to perform on-site setup and installation, the equipment shall be delivered and installed between the hours of 8:00am and 5:00pm, Eastern Standard Time to the Porter Neuroscience Research Center, Building 35 on the NIH Bethesda campus located at 35 Convent Drive, Bethesda, MD 20814.

8. Warranty Contractor shall warrant that the equipment for a minimum of twelve (12) months from the date of acceptance covering parts and labor.

9. Period of Performance:

Service and maintenance support covered under the warranty for the Base Period from the date of acceptance, and Five (5) Option Periods for an extended warranty coverage for services and maintence support.

10. Option(s): Five option periods for extended warranty coverage for service and maintenance support.