Attachment 1_Statement of Work Services updated_08_14_2020.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

STATEMENT OF WORK (SERVICES)

(SOW)

GENERAL INFORMATION

Title of Project:

Next Generation Sequencing (NGS) in Psychiatric Disorders

Statement of Need and Purpose:

The Human Genetics branch (HGB) at National Institute of Mental Health (NIMH) works on genetics basis of mood and anxiety disorders. HGB would like to sequence the DNA (exome sequencing and whole genome sequencing) and RNA (stranded RNA sequencing) obtained from individuals and their family members suffering with mood and anxiety disorders. Exome sequencing needs to be done on 140 samples, whole genome on 47 samples and RNA sequencing on 116 samples.

During the option period the program will require sequencing on DNA using exome sequencing on 70 samples and whole genome sequencing on 20 samples, and sequencing the RNA using RNA sequencing on 58 samples.

Background Information and Objective:

HGB investigates the genetic basis of mood and anxiety disorders. In this regard, we have been collecting the DNA and RNA from individuals and their family members with mood and anxiety disorders. In order to find the genes we need to sequencing the DNA using exome sequencing on 140 samples and whole genome sequencing on 47 samples, and sequencing the RNA using RNA sequencing on 116 samples. By using multiple techniques, we will be able to find the genes responsible for these devastating mental disorders.

Period of Performance:

We want the sequencing to start on September 1, 2020. The exome sequencing, whole genome sequencing and RNA sequencing should be completed by August 30th, 2021. The option period will begin September 1, 2021 through August 31, 2022

SCOPE OF WORK

General Requirements:

Independently and not as an agent of the Government, the Contractor shall furnish all the necessary services, qualified personnel, material, equipment, and facilities, not otherwise provided by the Government as needed to perform the Statement of Work below:

The company should do the following:

1. Perform quality control of the DNA and RNA.

2. Make the libraries

3. Perform paired end sequencing.

4. Perform sequencing

5. Deliver high quality sequence data (Q30>=80%)

6. Return 30X whole genome data on every sample submitted

7. Return 100X coverage for whole exome sequencing on every sample submitted

8. ~100 Million reads GB raw data for RNA sequencing for every sample submitted

Specific Requirements:

The company has to provide high quality paired end sequence data with Q30>=80%. The company has to give the fastq files generated from exome sequencing (100X coverage/sample), whole genome sequencing (30X coverage/sample) and stranded RNAsequencing (~100M reads). After analysis if HGB/NIMH finds the data is not of high quality they have resequence the samples.

LEVEL OF EFFORT:

Not applicable

GOVERNMENT RESPONSIBILITIES

The Bioinformatics team at HGB will analyze the fastq files and if the data is not of high quality (Q30>=80%) and if the amount of exome sequencing is not 100X coverage/sample, whole genome sequencing is not 30X coverage/sample and stranded RNAsequencing is not ~100M reads/sample, the company has to perform the QC, library and sequencing of the failed samples.

DELIVERY OR DELIVERABLES

1. Return the QC results

2. Deliver high quality sequence data (Q30>=80%)

3. Return 30X whole genome data on every sample submitted

4. Return 100X coverage for whole exome sequencing on every sample submitted

5. ~100 Million reads GB raw data for RNA sequencing for every sample submitted

6. Perform the QC, library preparation and sequencing for samples that failed in the initial analysis

7. Return any library or extra DNA or RNA remaining to HGB/NIMH

OTHER CONSIDERATIONS

Travel:

Key Personnel:

Not Applicable

Information System Security Plan:

Not applicable

Data Rights:

The DNA, RNA, the product of generated from these samples like the libraries, sequence data belongs to the Human Genetics Branch (HGB), National Institute of Mental Health (NIMH), NIH, Bethesda, MD – 20892

Section 508—Electronic and Information Technology Standards:

Publications and Publicity:

Confidentiality of Information: