Attachment 1 - Specifications.pdf

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Orthonairovirus Genome Synthesis Federal contract opportunity
Solicitation number
12805B22Q0511
Issued by
Department of Agriculture Agricultural Research Service Field Research Implementation and Information Delivery Plains Area

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Solicitation_12805B22Q0511.pdf PDF

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Minimum specifications

The following minimum specifications are required for each construct. The constructs must be full-length synthetic DNA fragments with 100% guaranteed sequence accuracy, free of any mutations or negative clones. GenBrick or equivalent method to ensure accuracy and length is required. Specific plasmid, promotor and other requirements are listed.

• SC1010-US Gene synthesis_US (limited release):T7_HHRz_CCHFV_SPU103_8 7 G3 Sm_HDV o Length: 1995bp

• SC1691-US-HT Express Cloning-USHT(limited release):T7_HHRz_CCHFV_SPU103_8 7 G3 Sm_HDV_pUC57 Plasmid Name: pUC57 Vector source:

o Default vector Cloning site: No preference/No preference Delivery:

o Standard 4 μg free of charge (1 μg for low-copy plasmid) o Delivery form: Freeze dried Desired buffer:

• SC1584 GenBrick synthesis:T7_HHRz_AIGV_AP92 seg L_HDV o Length: 12486bp"

• SC1017 Subcloning and gene synthesis:T7_HHRz_AIGV_AP92 seg L_HDV_pUC57-Brick o Plasmid Name: pUC57-Brick Vector source: Default vector Cloning site: EcoRV/EcoRV o Cloning direction: Opposite direction to promotor o Promotor: Amp

• SC1584 GenBrick synthesis:T7_HHRz_NSDV_Jilin seg L_HDV o Length: 1995bp

• SC1017 Subcloning and gene synthesis:T7_HHRz_NSDV_Jilin seg L_HDV_pUC57-Brick o Plasmid Name: pUC57-Brick Vector source: Default vector Cloning site: EcoRV/EcoRV o Cloning direction: Opposite direction to promotor o Promotor: Amp o Delivery form: Freeze dried Desired buffer: --

• SC1584 GenBrick synthesis:T7_HHRz_TAMV_YL16083 seg L_HDV o Length: 12486bp

• SC1017 Subcloning bundled with gene synthesis:T7_HHRz_TAMV_YL16083 seg L_HDV_pUC57-Brick o Plasmid Name: pUC57-Brick Vector source: Default vector Cloning site: EcoRV/EcoRV o Cloning direction: Opposite direction to promotor o Promotor: Amp o Delivery form: Freeze dried Desired buffer: --

• SC1584 GenBrick synthesis:T7_HHRz_ISKV_LEIV-315K seg L_HDV o Length: 12486bp

• SC1017 Subcloning bundled with gene synthesis:T7_HHRz_ISKV_LEIV-315K seg L_HDV_pUC57-Brick o Plasmid Name: pUC57-Brick Vector source: Default vector Cloning site: EcoRV/EcoRV o Cloning direction: Opposite direction to promotor o Promotor: Amp o Delivery form: Freeze dried Desired buffer: --

• SC1584 GenBrick synthesis:T7_HHRz_Songling_YC585 seg L_HDV o Length: 12323bp"

• SC1017 Subcloning bundled with gene synthesis:T7_HHRz_Songling_YC585 seg L_HDV_pUC57-Brick

• Plasmid Name: pUC57-Brick Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

• Promotor: Amp

• Delivery: Standard 4 μg free of charge (1 μg for low-copy plasmid)

• Delivery form: Freeze dried Desired buffer: --

• Required concentration:

SC1584 GenBrick synthesis:T7_HHRz_Yezo_HH003- 2020 seg L_HDV

• Length: 12465bp"

SC1017 Subcloning bundled with gene synthesis:T7_HHRz_Yezo_HH003- 2020 seg L_HDV_pUC57-Brick Plasmid Name: pUC57-Brick

• Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

• Promotor: Amp

• Delivery form: Freeze dried Desired buffer: --

SC1010 Gene synthesis:T7_HHRz_AIGV_AP92 seg M_HDV

• Length: 5738bp

SC1691 Express Cloning:T7_HHRz_AIGV_AP92 seg M_H D V_pUC57

• Plasmid Name: pUC57 Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

• Promotor: LacZ

• Delivery form: Freeze dried Desired buffer: --

SC1010 Gene synthesis:T7_HHRz_AIGV_AP92 seg S_HDV

• Length: 1981bp"

SC1691 Express Cloning:T7_HHRz_AIGV_AP92 seg S_H D V_pUC57

• Plasmid Name: pUC57 Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

• Promotor: LacZ

• Delivery form: Freeze dried Desired buffer: --

SC1010 Gene synthesis:T7_HHRz_NSDV_Jilin seg M_HDV

• Length: 5399bp"

SC1691 Express Cloning:T7_HHRz_NSDV_Jilin seg M_HDV_pUC57

• Plasmid Name: pUC57 Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

• Promotor: LacZ

• Delivery form: Freeze dried Desired buffer: --

SC1010 Gene synthesis:T7_HHRz_NSDV_Jilin seg S_HDV

• Length: 1912bp

SC1691 Express Cloning:T7_HHRz_NSDV_Jilin seg S_HDV_pUC57

• Plasmid Name: pUC57 Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

• Promotor: LacZ

• Delivery form: Freeze dried Desired buffer: --

SC1010 Gene synthesis:T7_HHRz_TAMV_YL16083 seg M_HDV

• Length: 4927bp"

SC1691 Express Cloning:T7_HHRz_TAMV_YL16083 seg M_HDV_pUC57

• Plasmid Name: pUC57 Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

• Promotor: LacZ

• Delivery form: Freeze dried Desired buffer: --

SC1010 Gene synthesis:T7_HHRz_TAMV_YL16083 seg S_HDV

• Length: 2370bp

SC1691 Express Cloning:T7_HHRz_TAMV_YL16083 seg S_HDV_pUC57

• Plasmid Name: pUC57 Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

• Promotor: LacZ

• Delivery form: Freeze dried Desired buffer: --

SC1010 Gene synthesis:T7_HHRz_Songling_YC585 seg M_HDV

• Length: 4657bp

SC1691 Express Cloning:T7_HHRz_Songling_YC585 seg M_H D V_pUC57

• Plasmid Name: pUC57 Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

• Promotor: LacZ

• Delivery form: Freeze dried Desired buffer: --

SC1010 Gene synthesis:T7_HHRz_Songling_YC585 seg S_HDV

• Length: 2203bp

SC1691 Express Cloning:T7_HHRz_Songling_YC585 seg S_H D V_pUC57

• Cloning direction: Opposite direction to promotor

• Promotor: LacZ

• Delivery form: Freeze dried Desired buffer: --

SC1010 Gene synthesis:T7_HHRz_Yezo_HH003-2020 seg M_HDV

• Length: 4603bp

"SC1691 Express Cloning:T7_HHRz_Yezo_HH003-2020 seg M_H D V_pUC57

• Plasmid Name: pUC57 Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

• Promotor: LacZ

• Delivery form: Freeze dried Desired buffer: --

SC1010 Gene synthesis:T7_HHRz_Yezo_HH003-2020 seg S_HDV

• Length: 2025bp

SC1691 Express Cloning:T7_HHRz_Yezo_HH003-2020 seg S_H D V_pUC57

• Plasmid Name: pUC57 Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

• Promotor: LacZ

• Delivery form: Freeze dried Desired buffer: --

SC1010 Gene synthesis:T7_HHRz_CCHFV_ArD8194 G1 Lg pt1_HDV

• Length: 6250bp

SC1691 Express Cloning:T7_HHRz_C CHFV_ArD8194 G1 Lg pt1_H D V_pUC57

• Plasmid Name: pUC57 Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

• Promotor: LacZ

• Delivery form: Freeze dried Desired buffer: --

SC1010 Gene synthesis:T7_HHRz_CCHFV_ArD8194 G1 Lg pt2_HDV

• Length: 6232bp"

SC1691 Express Cloning:T7_HHRz_C CHFV_ArD8194 G1 Lg pt2_H D V_pUC57

• Plasmid Name: pUC57 Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

• Promotor: LacZ

• SC1010 Gene synthesis:T7_HHRz_CCHFV_ArD8194 G1 seg M_HDV

• Length: 5708bp"

"SC1691 Express Cloning:T7_HHRz_C CHFV_ArD8194 G1 seg M_H D V_pUC57

• Plasmid Name: pUC57 Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

• Promotor: LacZ

• Delivery form: Freeze dried Desired buffer: --

SC1010 Gene synthesis:T7_HHRz_CCHFV_ArD8194 G1 seg S_HDV

• Length: 2008bp

SC1691 Express Cloning:T7_HHRz_C CHFV_ArD8194 G1 seg S_H D V_pUC57

• Plasmid Name: pUC57 Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

• Promotor: LacZ

• Delivery form: Freeze dried Desired buffer: --

• Required concentration:"

SC1010 Gene synthesis:T7_HHRz_CCHFV_UG3010 G2 Lg pt1_HDV

• Length: 6250bp"

SC1691 Express Cloning:T7_HHRz_C CHFV_UG3010 G2 Lg pt1_H D V_pUC57

• Plasmid Name: pUC57 Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

• Promotor: LacZ

• Delivery form: Freeze dried Desired buffer: --

SC1010 Gene synthesis:T7_HHRz_CCHFV_UG3010 G2 Lg pt2_HDV

• Length: 6200bp"

SC1691 Express Cloning:T7_HHRz_C CHFV_UG3010 G2 Lg pt2_H D V_pUC57

• Plasmid Name: pUC57 Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

• Promotor: LacZ

• Delivery form: Freeze dried Desired buffer: --

SC1010 Gene synthesis:T7_HHRz_CCHFV_UG3010 G2 seg M_HDV

• Length: 5710bp"

SC1691 Express Cloning:T7_HHRz_C CHFV_UG3010 G2 seg M_H D V_pUC57

SC1010 Gene synthesis:T7_HHRz_CCHFV_UG3010 G2 seg S_HDV

• Length: 1962bp"

"SC1691 Express Cloning:T7_HHRz_C CHFV_UG3010 G2 seg S_H D V_pUC57

• Plasmid Name: pUC57 Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

• Promotor: LacZ

• Delivery form: Freeze dried Desired buffer: --

"SC1010 Gene synthesis:T7_HHRz_CCHFV_SPU103_8 7 G3 Lg pt1_H

• Length: 6250bp

SC1691 Express Cloning:T7_HHRz_C CHFV_SPU103_87 G3 Lg pt1_H_pUC57

• Plasmid Name: pUC57 Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

• Promotor: LacZ

• Delivery form: Freeze dried Desired buffer: --

SC1010 Gene synthesis:T7_HHRz_CCHFV_SPU103_8 7 G3 Lg pt2_H

• Length: 6230bp"

SC1691 Express Cloning:T7_HHRz_C CHFV_SPU103_87 G3 Lg pt2_H_pUC57

• Plasmid Name: pUC57 Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

• Promotor: LacZ

• Delivery form: Freeze dried Desired buffer: --

SC1010 Gene synthesis:T7_HHRz_CCHFV_SPU103_8 7 G3 Md_HDV

• Length: 5686bp"

SC1691 Express Cloning:T7_HHRz_C CHFV_SPU103_87 G3 Md_H D V_pUC57

• Plasmid Name: pUC57 Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

• Promotor: LacZ

• Delivery form: Freeze dried Desired buffer: --

SC1010 Gene synthesis:T7_HHRz_CCHFV_C-68031 G4 Lg pt1_HDV

• Length: 6250bp"

SC1691 Express Cloning:T7_HHRz_C CHFV_C-68031 G4 Lg pt1_H D V_pUC57

• Promotor: LacZ

• Delivery form: Freeze dried Desired buffer: --

SC1010 Gene synthesis:T7_HHRz_CCHFV_C-68031 G4 Lg pt2_HDV

• Length: 6240bp"

SC1691 Express Cloning:T7_HHRz_C CHFV_C-68031 G4 Lg pt2_H D V_pUC57

• Plasmid Name: pUC57 Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

• Promotor: LacZ

• Delivery form: Freeze dried Desired buffer: --

SC1010 Gene synthesis:T7_HHRz_CCHFV_C-68031 G4 seg M_HDV

• Length: 5689bp"

SC1691 Express Cloning:T7_HHRz_C CHFV_C-68031 G4 seg M_H D V_pUC57

• Plasmid Name: pUC57 Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

• Promotor: LacZ

• Delivery form: Freeze dried Desired buffer: --

SC1010 Gene synthesis:T7_HHRz_CCHFV_C-68031 G4 seg S_HDV

• Length: 1994bp"

"SC1691 Express Cloning:T7_HHRz_C CHFV_C-68031 G4 seg S_H D V_pUC57

• Plasmid Name: pUC57 Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

• Promotor: LacZ

• Delivery: Standard 4 μg free of charge (1 μg for low-copy plasmid)

• Delivery form: Freeze dried Desired buffer: --

SC1010 Gene synthesis:T7_HHRz_CCHFV_Drosdov G5 Lg pt1_HDV

• Length: 6250bp"

SC1691 Express Cloning:T7_HHRz_C CHFV_Drosdov G5 Lg pt1_H D V_pUC57

• Plasmid Name: pUC57 Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

• Promotor: LacZ

"SC1010 Gene synthesis:T7_HHRz_CCHFV_Drosdov G5 Lg pt2_HDV

• Length: 6223bp

SC1691 Express Cloning:T7_HHRz_C CHFV_Drosdov G5 Lg pt2_H D V_pUC57

• Plasmid Name: pUC57 Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

• Promotor: LacZ

• Delivery form: Freeze dried Desired buffer: --

SC1010 Gene synthesis:T7_HHRz_CCHFV_Drosdov G5 seg M_HDV

• Length: 5686bp

SC1691 Express Cloning:T7_HHRz_C CHFV_Drosdov G5 seg M_H D V_pUC57

• Plasmid Name: pUC57 Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

• Promotor: LacZ

• Delivery form: Freeze dried Desired buffer: --

SC1010 Gene synthesis:T7_HHRz_CCHFV_Drosdov G5 seg S_HDV

• Length: 1993bp

SC1691 Express Cloning:T7_HHRz_C CHFV_Drosdov G5 seg S_H D V_pUC57 Plasmid Name: pUC57

• Vector source: Default vector Cloning site: EcoRV/EcoRV

• Cloning direction: Opposite direction to promotor

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