Attachment 1 - Specifications and Installation Instructions.pdf

Attachment 1 – Specifications and Installation Instructions

US Department of Agriculture (USDA)

Contents Project General Information

1.0 Scope of Work

2.0 Background

Contractor Requirements

3.0 Technical Requirements / Tasks

4.0 Government Furnished

5.0 Deliverables / Schedule

6.0 Travel

7.0 Contractor’s Key Personnel

8.0 Security Requirements ...................................................................................................................... Error! Bookmark not defined.

9.0 Data Rights ........................................................................................................................................ Error! Bookmark not defined.

10.0 Section 508 – Electronic and Information Technology Standards .................................................. Error! Bookmark not defined.

Attachment ............................................................................................................................................. Error! Bookmark not defined.

Project Purchase of DASGIP Four-Vessel Parallel Bioreactor Upgrade

General Information

1.0 Scope of Work

Vendor is to deliver and install a new Eppendorf DASGIP four-vessel parallel bioreactor upgrade on an existing system.

2.0 Background

DASGIP Parallel Bioreactor Systems for R&D and process development in microbiology allow for advanced bioprocess control and automation. Parallel processing, precise control of all relevant parameters (e.g., temperature, pH, stirring, aeration) and numerous automation features (e.g., controlled feeding and antifoam addition) result in improved process development of microbial production of desired bioproducts. The USDA Agricultural Research Service (ARS) in Peoria, Illinois currently has an Eppendorf DASGIP eight-vessel parallel bioreactor system that was purchased in 2006 and is no longer serviceable. In addition, a newer model Eppendorf DASGIP four-vessel parallel bioreactor system (200 ml-1.0 L) was purchased in 2022. There is a need to upgrade this newer unit with an additional four-vessel parallel bioreactor system so that up to eight parallel vessels can be utilized simultaneously using the same DASware control system.

Contractor Requirements

3.0 Technical Requirements / Tasks

The Eppendorf DASGIP four-vessel parallel bioreactor upgrade must be seamlessly integrated into the current system with identical, or at least fully compatible components, as determined by manufacturer specifications and recommendations. This is required for reproducibility of replicates with all eight vessels and to ensure DASware software control systems can properly communicate with the various components.

Technical Requirements-

1. Configurable DASGIP System (DG-System) consisting of the following components:

a. DASware® control Upgrade, for DASGIP® system, incl. database update and licenses, adding 4 vessels

(76DGCS+4)

b. DASGIP® Vessel, SR0700ODLS, 200 mL – 1.0 L, L-Sparger, overhead drive, 2 Rushton-type impellers, DASGIP® Bioblock (4x 76SR0700ODLS)

c. DO Sensor (Optical), Hamilton®, for 1 vessel, O.D. 12 mm, L 220 mm (4x 76DGPOHVF220)

d. Level/Foam Sensor, stainless steel with PFA coating, including compression fitting, for 1 vessel, Pg 13.5, L

220 mm, Li 20 – 160 mm (4x 76DGLVL220)

e. Exhaust Condenser, including accessories for 1 vessel, Pg 13.5, O.D. 30 mm (4x 76DGCOND30)

f. Triple Port, including liquid feed connector assembly, for 1 vessel, Pg 13.5 (4x 76DGTRIP)

g. DASGIP® TC4SC4 Temperature and Agitation Control Module, without sensors, for DASGIP® Bioblock and overhead drives (TC4SC4B), for 4 vessels (76DGTC4SC4B)

h. Overhead Drive, RE40, 100 – 1600 rpm, digitally encoded, for 1 vessel (4x 76DGRE40)

i. DASGIP® Bioblock, 4-position heating/cooling block, max. temp. 99 °C, for 4 vessels (76DGTBLOCK)

j. Accessories, for DASGIP® TC4SC4 (76DGTCSCUM)

k. Accessories, for DASGIP® stirrer assembly (76DGSAUM)

l. DASGIP® PH4PO4L Monitoring Module, for 4 vessels, without sensors, pH and DO with level/anti foam option (76DGPH4PO4L)

m. Sensor Cable, for analog pH/Redox sensors, for 1 vessel, with plug type AK9 (4x 76DGPHRDAK9)

n. Sensor Cable, for optical DO sensors, for 1 vessel, with plug type VP8 (4x 76DGPOVP8)

o. Foam/Level Cable, for connecting a level sensor to a DASGIP® module, for 1 vessel (4x 76DGLVLC)

p. Accessories, for DASGIP® PHPO (76DGPHPOUM)

q. DASGIP® MF4 Gassing Module, for 4 vessels, mass flow controller, 10 – 300 sL/h, 10 – 180 sL/h CO2

(76DGMF4F300)

r. Accessories, for DASGIP® MX4/4(H), MX4/1(H), MF4 (76DGMXMFUM)

s. DASGIP® MP8 Multi Peristaltic Pump Module, for 8 feeds, without feed lines and addition bottles (2x

76DGMP8)

t. Feed Line Set PTFE, for DASGIP® MP8, 8 tubes including addition bottles, L 1.0/0.07/3.0 m, I.D. 0.5/0.8 mm (2x 76DGMP8FL05P13)

u. Accessories, for DASGIP® MP4-MP8 (76DGMPUM)

v. DASGIP® CWD4 Cooling Water Distribution Unit, including connection cable, for 4 condenser ports

(DASGIP® CWD4) (76DGCWD4)

w. Accessories, for DASGIP® CWD4, for 4-fold system (76DGCWD4UM)

x. Inline Water Filter, for parallel DASGIP® Bioblock or benchtop systems, including accessories for 4-fold or 8-fold systems (76DGIWF)

y. Bracket for Cables/Tubes, for DASGIP® Bioblock and cooling water distribution, 1 long and 1 short pipe

(76DGCBLS)

z. Installation Material, for a DASGIP® system, for 4 vessels (76DGINMAT4)

2. Uninterruptible Power Supply, for DASGIP®/DASbox® Mini Bioreactor System, 115 V (76DGUPSU1)

3. Startup Assistance, for a 4-fold DASGIP® system, US (76DGCOMQ4US)

4. Zone 2 Travel Charge (901511060)

5. Transportation and Transportation Insurance, 4-fold DASGIP® system (76DGTT4)

6. Packing Material, for a 4-fold DASGIP® system (76DGPC4)

7. DO Sensor (Optical), Hamilton® VisiFerm™, autoclavable, O.D. 12 mm, H0 cap, L 225 mm (4x 78108059)

8. pH Sensor, Hamilton® EasyFerm® Plus, autoclavable, O.D. 12 mm, L 225 mm (4x 78103230)

9. Installation and integration into existing Eppendorf DASGIP four-vessel parallel bioreactor system

10. One year warranty

4.0 Government Furnished

Government will supply a standard loading dock to accept delivery of the unit. Government-furnished support for installation is limited to security access to the equipment in the NCAUR facility, adequate working space, heat, light, ventilation, air conditioning, electric current and outlets for the use of company employees. Water and common solvents will also be made available for installation and validation methods.

5.0 Deliverables / Schedule

Key Deliverables

Item No. Deliverable Objective Due 1 Delivery and

Installation To set up and validate functionality of new Eppendorf DASGIP four-vessel parallel bioreactor system upgrade with existing system

No later than 120 calendar days after contract award.

6.0 Travel

Travel is required for installation and training.

7.0 Contractor’s Key Personnel

Installation must be performed by qualified technicians authorized by Eppendorf to work on DASGIP parallel bioreactor systems.