Attachment 1 - PWS.pdf
PDF 172 KB Posted
- Attached to
- Whole Genome Resequencing Federal contract opportunity
- Solicitation number
- 12805B23Q0776
About this file
This performance work statement outlines whole genome resequencing and RNA sequencing services required by the Agricultural Research Service. The contractor will provide high quality DNA and RNA sequencing data for 100 bulked mutant mapping populations and 200 total RNA samples to identify genes responsible for altered high temperature tolerance and male sterile mutants in sorghum. Sequencing deliverables include over 10Gb of data per DNA sample and over 12Gb of data per RNA sample. The related federal contract opportunity is solicited by the Department of Agriculture Agricultural Research Service Plains Area to provide these whole genome resequencing services. No pricing, response dates, or other contract terms are included in the file.
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| File | Type | Posted |
|---|---|---|
| Sol_12805B23Q0776.pdf |
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Text version
Performance Work Statement (PWS)
Project: Whole genome resequencing and RNAseq Service Agreement
GENERAL INFORMATION
1.0 Background and Purpose: Whole genome sequencing for 100 bulked mutant mapping populations is requested to identify genes responsible for altered high temperature tolerance and male sterile mutants discovered by two CRIS projects (3096-21000-021-00D and 3096-21000-022- 00D). These genes are critical to develop high yield and stress tolerant sorghum hybrids. RNAseq for 200 total RNA samples is requested to characterize the mechanism of impacts of high temperature stress on sorghum panicle, pollen and seed development in relation to yield formation.
This service also provides a wide range of targets to improve sorghum yield under heat stress.
2.0 Definitions: N/A
CONTRACTOR REQUIREMENTS
3.0 Technical Requirements/Tasks: We will provide total DNA and total RNA samples to the company for WGS and RNAseq. We are requesting the service company to deliver high quality DNA sequencing and RNA sequencing data in a timely manner.
4.0 Deliverable / Schedule:
o Promptly when the total DNA and total RNA samples are submitted.
5.0 Government Furnished: High quality genome DNA and total RNA samples on Dry Ice
6.0 Travel: No travel needed
7.0 Contractor’s Key Personnel: Sale’s rep and project manager
8.0 Security Requirements: None
9.0 Data Rights: Confidential to ARS. The sequencing data should not released to third party.
10.0 Section 508 – Electronic and Information Technology Standards: Operational software maintained to operate under current Windows operating system.
11.0 Performance Requirement Measures:
Whole genome resequencing of total DNA samples submitted: Over 10 Gb high quality DNA sequencing data per sample.
RNAseq of the total RNA samples submitted: Over 12 Gb high quality RNA DNA sequencing data per sample.
6.0 Travel: No travel needed
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