Attachment 1 - PWS.pdf

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Attached to
Genomic Sequencing Federal contract opportunity
Solicitation number
12805B23Q0697
Issued by
Department of Agriculture Agricultural Research Service Field Research Implementation and Information Delivery Plains Area

About this file

This performance work statement outlines genomic sequencing services required by the USDA Agricultural Research Service Plains Area. The contractor will extract and quantify DNA from peanut seed samples provided by the government and conduct whole genome sequencing via the Khufu genomic analysis platform. The contractor must then determine any disease resistance, high oleic, or other traits present and provide the percentage of recurrent parent background. The contractor must be able to deliver hapmap formatted genotyping results and QTL-seq analysis reports on significant genetic loci. The number of peanut samples sequenced per year will vary depending on submissions from the government customer.

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Performance Work Statement

Project: Genomic Sequencing with Khufu Genomic Analysis Software

GENERAL INFORMATION

1.0 Scope of Work: PSGRU requires a service provider for the sequencing of DNA from biological samples to support research activities in peanut genetics. These services are required on request only. The number of samples processed per year will vary.

2.0 Background: The Khufu platform provides whole genome sequencing at low coverage along with data analysis that enables the PSGRU to rapidly select for traits of interest in peanut and determination of percent recurrent parent background, saving resources that would otherwise be expended testing for performance in the field at early generations of breeding line development.

CONTRACTOR REQUIREMENTS

3.0 Technical Requirements/Tasks:

1. DNA extraction and quantification from chips of peanut seeds.

2. WGS via Khufu platform.

3. Determination of what traits are present (i.e., disease resistance, high oleic, etc.)

4. Percent recurrent parent background.

5. Data interpretation and consultation.

4.0 Government Furnished: The PSGRU will provide peanut samples in the form of seed chips.

5.0 Deliverables / Schedule: The delivery schedule will vary depending on submission of samples by PSGRU. The service provider must be able to provide PSGRU with the following services, among others:

o For Genotyping Services, the results are in hapmap format.

o For QTL-seq Services, the results will be provided in a report of the analyzed dataset for significant genetic loci.

CONTRACTOR REQUIREMENTS
3.0 Technical Requirements/Tasks:

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