Requirements_for_Sourcing_12-Capillary_dsDNA_RNA_Fragment_Analysis_System_2014DEC22.docx

Requirements for dsDNA/RNA Fragment Analysis System Instrument must meet the mandatory requirements specified below. Bidders MUST respond with complete specifications and/or descriptive literature to demonstrate that all requirements are met.

Specifications

1) Must be able to perform simultaneous, parallel injection and separation of at least 12 dsDNA and/or RNA samples at once from a 96-well sample plate

2) Must be able to provide analysis of at least three 96-well sample plates (288 samples) unattended without any user intervention or additional robotics/liquid handling required

3) Must have automated flushing of the capillaries between every injection cycle, to eliminate sample carryover

4) Must have the ability to perform two discrete application methods (dsDNA and/or RNA analysis) in succession without the need for stopping in-progress instrument operation

5) Must have demonstrated sensitivity of at least 5 pg/L

6) Must be able to separate dsDNA fragments from 1 bp to 40,000 bp

7) Must be able to analyze dsDNA amplified without the use of fluorescently labeled primers

8) Must utilize a capillary array format with the ability to use capillary arrays of different lengths

9) Must be able change 96-well sample plates without the need for stopping in-progress instrument operation

10) Must have a data analysis software package capable of sizing, quantification and report generation

11) Must use LED based fluorescence with excitation at 470 nm

12) Must come with a computer and all accessories