Amendment 1 75N95020Q00165.pdf

OMB No. 0990-0115

AMENDMENT OF SOLICITATION/MODIFICATION OF CONTRACT

1. CONTRACT ID CODE

PAGE

OF PAGES

2. AMENDMENT/MODIFICATION NO.

One (1)

3. EFFECTIVE DATE

09/09/2020

4. REQUISITION/PURCHASE REQ. NO.

5. PROJECT NO. (if applicable)

6. ISSUED BY CODE

7. ADMINISTERED BY (If other than Item 5) CODE

National Institutes of Health National Institute on Drug Abuse Bethesda, MD 20892-7511

8. NAME AND ADDRESS OF CONTRACTOR (No., street, city, county, State and ZIP Code)

9A. AMENDMENT OF SOLICITATION NO.

75N95020Q00165

X

9B. DATED (SEE ITEM 11)

09/02/2020

10A. MODIFICATION OF CONTRACT/ORDER NO.

CODE

FACILITY CODE

10B. DATED (SEE ITEM 13)

11. THIS ITEM ONLY APPLIES TO AMENDMENTS OF SOLICITATIONS

X The above numbered solicitation is amended as set forth in item 14. The hour and date specified for receipt of Offers X is extended, is not extended.

Offers must acknowledge receipt of this amendment prior to the hour and date specified in the solicitation or as amended, by one of the following methods:

(a) By completing Items 8 and 15 and returning _____ copies of the amendment; (b) By acknowledging receipt of this amendment on each copy of the offer submitted; or By separate letter or telegram which includes a reference to the solicitation and amendment numbers. FAILURE OF YOUR ACKNOWLEDGMENT TO BE RECEIVED AT THE PLACE DESIGNATED FOR THE RECEIPT OF OFFERS PRIOR TO THE HOUR AND DATE SPECIFIED MAY RESULT IN REJECTION OF YOUR OFFER. If by virtue of this amendment you desire to change an offer already submitted, such change may be made by telegram or letter, provided each telegram or letter makes reference to the solicitation and this amendment, and is received prior to the opening hour and date specified.

12. ACCOUNTING AND APPROPRIATION DATA (if required)

13. THIS ITEM APPLIES ONLY TO MODIFICATIONS OF CONTRACTS/ORDERS,

IT MODIFIES THE CONTRACT/ORDER NO. AS DESCRIBED IN ITEM 14.

A. THIS CHANGE ORDER IS ISSUED PURSUANT TO: (Specify authority) THE CHANGES SET FORTH IN ITEM 14 ARE MADE IN THE CONTRACT ORDER NO. IN ITEM 10A.

B. THE ABOVE NUMBERED CONTRACT/ORDER IS MODIFIED TO REFLECT THE ADMINISTRATIVE CHANGES (such as changes in paying office, appropriation date, etc.) SET FORTH IN ITEM 14, PURSUANT TO THE AUTHORITY OF FAR 43.103(b).

C. THIS SUPPLEMENTAL AGREEMENT IS ENTERED INTO PURSUANT TO AUTHORITY OF: FAR 1-602-1 and FAR 43.103(a) D. OTHER (Specify type of modification and authority)

E. IMPORTANT: Contractor __ is not, __ is required to sign this document and return __ copies to the issuing Office.

14. DESCRIPTION OF AMENDMENT/MODIFICATION (Organized by UCF section headings, including solicitation/contract subject matter where feasible.)

PURPOSE: To answer vendor questions and extend due date for responses.

Except as provided herein, all terms and conditions of the document referenced in item 9A or 10A, as heretofore changed, remains unchanged and in full force and effect.

16A. NAME AND TITLE OF CONTRACTING OFFICER

15B. CONTRACTOR/OFFEROR

BY ______________________________________________

(Signature of person authorized to sign)

15C. DATE SIGNED

16B. UNITED STATES OF AMERICA

BY _______________________________________________

(Signature of Contracting Officer)

16C. DATE SIGNED

NSN 7540-01-152-8070 30-105 STANDARD FORM 30 (REV 10-83)

PREVIOUS EDITION NOT USABLE Prescribed by GSA

FAR (48 CFR) 53.214(a)

Amendment 1 75N95020Q00165

1). What fluorophores will be imaged?

Mammalian embryos, ES cells, iPS cells, other cell lines such as 293T, COS7, CHO cells

2). Is a white light source where the fluorescence is controlled by filter cubes preferred, or a filter wheel based configuration?

Filter cubes preferred

3). Please specify the objectives, ie magnification, correction (ex plan apo, plan fluor, etc), and which ones are dedicated to phase versus coverslip only imaging.

×4, ×10, ×20, ×40 Phase contrast and ×4, ×10, ×20, ×40 DIC (Relief contrast)

5). What sample vessels will be imaged?

3.5 cm dish, 6 cm dish, 10 cm dish, 4-well plate (for IVF), 6-well plate, 12-well plate, 24-well plate, 96-well plate etc.

6). The specifications include 2 decks. Are you using both tiers in this configuration? Are they both being used for filter turrets? Or filter wheels? Or do you just want the future option to stage up to 2 tiers, but this configuration is a single tier?

Filter turrets and, magnification changer (may be 2 Filter turrets, or filter turrets and second camera in the future)

7). Is a piezo needed?

No

8). Is incubation needed?

Yes

9). Will stitching/tiling be one of the applications with this microscope?

No

10). Will the user use 2x3 slides for Marmoset tissue?

No