36A77620Q0137-002.docx

BioTek Synergy™ Hybrid Multi-mode Microplate Reader or Equal Salient Characteristics Project: VAPAHCS Research VMU Features, Design and Construction Common to Hybrid Multi-mode Microplate Readers:

· The hybrid multi-mode microplate reader shall include the following detection modes: top and bottom fluorescence intensity, UV-visible absorbance, luminescence, fluorescence polarization, TRF & TR-FRET.

· The hybrid multi-mode microplate reader shall include temperature control to 45 ̊C and Shaking.

· The hybrid multi-mode microplate reader shall include one filter cube with filters and data analysis software.

· The data analysis software shall offer advanced reader control functionality, powerful data analysis and flexible exporting/reporting tools combined in an easy-to-use software package.

· The data analysis software shall include software upgrades to meet windows operation system updates through the life of equipment.

· The hybrid multi-mode microplate reader shall be a GSA labeled product.

· The hybrid module microplate reader shall include the dual reagent injector module accessory.

· The dual reagent injector module shall include two (2) syringes, two (2) reagent bottles, holders and inlet tubing, and feedthrough assembly with straight dispense tips.

Warranty Common to Hybrid Multi-mode Microplate Readers:

· The vendor shall provide a warranty of twelve months of parts and labor.

Certifications, Environmental Impact and Testing Common to Hybrid Multi-mode Microplate Readers:

· CE and TUV marked. RoHS compliant.

Critical Characteristics Unique to Hybrid Multi-mode Microplate Readers:

· Absorbance:

· Light Source: Xenon Flash

· Detector: photodiode

· Wavelength selection: monochromator

· Wavelength range: 230-999 nm, 1 nm increments

· Monochromator bandwidth: 4 nm, 8 nm

· Dynamic range: 0 – 4.0 OD

· Resolution: .0001 OD

· Pathlength correction: yes

· Monochromator wavelength accuracy: ±2 nm

· Monochromator wavelength repeatability: ±0.2 nm

· OD accuracy: <1% at 2.0 OD; <3% at 3.0 OD

· OD linearity: <1% from 0 to 3.0 OD

· OD repeatability: <.5% at 2.0 OD

· Stray light: 0.03% at 230 nm

· Reading speed (kinetic): 96 wells: 11 seconds; 384 wells: 22 seconds

· Fluorescence Intensity:

· Light Source: Xenon Flash

· Detector: PMT for monochromator system; PMT for filter system

· Wavelength selection: Quad monochromators (top/bottom); Filters (top)

· Wavelength range: Monochromators: 250-700 nm; Filters: 200-700 nm

· Monochromator bandwidth: Fixed, 16 nm

· Dynamic range: 7 decades

· Sensitivity:

· Filters: Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate);

· Quad Monochromator:Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) – top;

· Fluorescein 4 pM (0.4 fmol/well, 384-well plate) – bottom

· Reading speed (kinetric): 96 wells: 11 seconds; 384 wells: 22 seconds

· Luminescence:

· Wavelength range: 300-700 nm

· Dynamic range: >6 decades

· Sensitivity:

· Monos: 20 amol ATP (flash)

· Filters 10 amol ATP (flash), 100 amol (glow)

· Fluorescence Polarization:

· Light source: Xenon flash

· Detector: PMT

· Wavelength selection: Filters

· Wavelength range: 280-700 nm

· Sensitivity: 1.2 mP standard deviation at 1 nm fluorescein

· Time-Resolved Fluorescence:

· Light source: Xenon Flash

· Detector: PMT

· Wavelength selection: Quad monochromators (secondary mode); Filters (top)

· Wavelength range: Filters: 200-700 nm

· Sensitivity:

· Filters: Europium 40 fM (4 amol/well, 384-well plate);

· Monos: Europium 1200 fM (120 amol/well, 384-well plate)

· Reagent Dispensers:

· Supported detection modes: All modes

· Number: 2 syringe pumps

· Supported labware: 6- to 384-well microplates, Petri dishes

· Dead volume: 1.1 mL with back flush

· Dispense volume: 5 – 1000 µL in 1 µL increment

· Dispense accuracy: ± µL or 2%

· Dispense precision: <2% at 50-200 µL