20-003740 - Nikon Confocal Upgrade PD Usdin_revised.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: The NIMH Systems Neuroscience Imaging Resource (SNIR) requires an upgrade of its existing Nikon confocal microscope system to ensure that it better meet the needs of users by acquiring high quality images much more quickly. The upgrade includes a new resonant scanner, 4 channel detector, 730nm laser, piezo stage, and silicone oil objective.

2. Background Information and Objective: Imaging brain sections using confocal microscopy is an essential step for experiments that are being carried out by many basic science investigators within the NIMH intramural program. The SNIR supports this by managing shared equipment used by multiple investigators. One of the microscopes managed by the SNIR is a Nikon system that has a relatively slow confocal scan head and a two-channel detector. Image acquisition takes a long time on this microscope which limits the work that can be done. The purpose of this acquisition is to upgrade the existing Nikon system so that acquisition is much faster, so that longer wavelength dyes such as Cy7 can be imaged, and so that high resolution can be obtained deeper into histological material. This will allow more users to collect data using it and for each user to collect better quality and more data.

3. Generic Name of Product: Upgrade laser scanning confocal microscope.

4. Purchase Description:

i) MHA50400 A1R HD25 Scanhead and Controller

ii) 99444 A1-DU4G HD Compatible Detector Unit with 2 GaAsP and 2 high sensitivity PMTs

a) 99343 Custom Ti2 Filter SetDichroic: 495Emitter: 447/57

b) 99344 Custom Ti2 Filter SetDichroic: 562Emitter: 521/42 1

c) 99345 Custom Ti2 Filter SetDichroic: 652Emitter: 600/45Emitter: 650LP 1

d) MHV55010 A1-Adapter Set for Ti Inverted Microscope

e) MXA22094 A1 Ti Interlock Cable

f) MHF4550 LU-A1-A-A1 Connection Kit

iii) 730nm Laser Unit

a) 99238 LUNF 730 System for A1; V2

b) MHV56000 A1-HD25 Optional Laser Input Port

iv) MRD73400 CFI60 Plan Apochromat Lambda S 40xC Silicone Oil Immersion Objective

v) Stage with Piezo insert

a) 91989 Ti/Ti2 PIEZO STAGE INSERT W/ USB CNTRLR

b) 91997 UNIVERSAL HOLDER FOR PIEZO STAGE

c) 91998 MULTIPLE SLIDE HOLDER FOR PIEZO STAGE

d) MEC56120 TI2-S-SE-E MOTORIZED STAGE WITH ENCODER

e) MEF55705 TI2-S-JS JOYSTICK

5. Salient characteristics:

High Definition Resonant Scanner with 25mm diagonal field of view capable of 1024x1024 resolution or up to 720 frame per second compatible with Ti2e frame and Nikon Elements software

4 Channel Detector with 2 GaAsP and 2 High Sensitivity PMTs, 3 x 6 position filter wheels compatible with Ti2e frame and Nikon Elements software

XY stage with Piezo Z insert with 220 micron travel range compatible with Ti2e frame and Nikon Elements software

730 nm laser combined with far red enhanced detector, compatible with Nikon LUN-4 4-laser unit, Ti2e frame and Nikon Elements software

40x Silicone Oil Immersion Objective Lens, NA 1.25, W.D. 0.30mm, F.O.V. 25mm, DIC, Correction Collar, compatible with Nikon Ti2e frame

6. Quantity: One

7. Delivery Date: 60-90 days following the award of the contract

8. Period of Performance: All items fully warrantied for one year. Equipment is expected to be fully functional for greater than 5 years with minor service, as required.

Maintenance service after one year may be purchased as an annual contract or on an as needed basis.

9. Option(s): None