1125464 Samac SOW OmniC.docx

DOCX document 61 KB Posted

Attached to
Alfalfa Genome Assembly Federal contract opportunity
Solicitation number
12505B24Q0131
Issued by
Department of Agriculture Agricultural Research Service Field Research Implementation and Information Delivery Midwest Area

About this file

The provided document is a Statement of Work (SOW) for an Omni-C analysis of eight alfalfa genomes to support long-range genome mapping. The SOW outlines the scope of work, which involves the service provider performing Dovetail Omni-C assays on alfalfa leaf samples provided by the Plant Science Research Unit at the University of Minnesota. The goal is to obtain long-range mapping data to accurately assemble the four haplotypes of the alfalfa genome for eight unique alfalfa genotypes. The SOW specifies the technical requirements, including constructing a sufficient number of Omni-C libraries, sequencing each library to a target depth, and delivering the results files electronically. The service provider is solely responsible for the entire service, and all data generated belongs to the government. The required delivery schedule is 7 weeks from the receipt of samples.

The related federal contract opportunity is a Request for Quote (RFQ) solicitation for the Omni-C analysis of the eight alfalfa genomes, in accordance with the provided SOW. The solicitation is issued as a 100% Total Small Business Set-Aside, with the applicable NAICS code of 541714 (Research and Development in Biotechnology) and a small business size standard of under 1,000 employees. The solicitation is issued by the Department of Agriculture Agricultural Research Service Field Research Implementation and Information Delivery Midwest Area, and all quotes must be submitted by 11:00 AM CT on May 07, 2024.

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Statement of Work (SOW) Project: Omni-C analysis of eight alfalfa genomes for long-range mapping

GENERAL INFORMATION

1.0 Scope of Work:

The service provider will perform Dovetail® Omni-C® Assays from alfalfa leaf samples provided by the Plant Science Research Unit located at the University of Minnesota, St. Paul, MN. The goal of the ARS project is to obtain long range mapping data to accurately assemble the four haplotypes of the alfalfa genome for eight unique alfalfa genotypes.

2.0 Background:

Developing genomic resources for alfalfa has lagged behind other crop species. One goal of this project is to assemble the genomes of diverse alfalfa plants and carry out comparisons to identify key genes responsible for agronomic traits important to alfalfa farmers. The highly heterogeneous and tetraploid nature of alfalfa makes assembly of genomes very complex. Long range mapping by Omni-C is required to accurately assemble genomes.

CONTRACTOR REQUIREMENTS

2.0 Technical Requirements/Tasks:

Young leaf material from eight plants will be shipped to the service provider on dry ice. The service provider will perform these specific tasks:

1. Construct a sufficient number of Omni-C libraries to generate 15x coverage per haplotype of genome.

2. Sequence each Omni-C library to a target depth of 15X per haplotype of PE150 reads.

3. Deliver the results files to the Customer electronically via secure FTP. The Results shall consist of: QC report and Raw ILMN data from deep sequencing the Omni-C library

Delivery schedule: 7 weeks from the receipt of samples.

3.0 Government Furnished:

The service provider is solely responsible for the entire service. There will be no support or use of federal facility by the service provider. The service provider will submit a report to end user at the end of the completion of the service.

4.0 Deliverables / Schedule:

All data generated by the vendor will be submitted to Deborah Samac at debby.samac@usda.gov.

5.0 Travel:

No Travel requirements

6.0 Contractor’s Key Personnel:

Not applicable

7.0 Security Requirements:

Not applicable

8.0 Data Rights:

All the data generated by the vendor belongs to the government.

9.0 Section 508 – Electronic and Information Technology Standards:

Not applicable.

File details come from the government source that posted it. Updated .