Specs_TEM_Micro_-1.docx

REV - 1 08/28/2019

Transmission Electron microscope

Specifications:

Resolution: 0.20 nm@ 100kV

Dual Lens Modes: Standard configuration provides High Contrast (HC) and High Resolution (HR) imaging modes by utilizing a unique compound objective lens system to alter focal length and Cs values for optimized operation under a wide range of conditions /samples. Switching between modes is done via software control

Digital Operation: Microscope setup, electrical alignments and sample search procedures are conducted under normal room light conditions utilizing a high speed digital navigation camera with a standard computer display. An integrated high-resolution camera is included whereby final image collections are obtained in the same. For teaching and presentation purposes, operations (from setup to final image capture) can be displayed on a large wall monitor, at a remote location, or recorded to video using common PC accessories.

Turbo-Molecular Pumped (TMP) Vacuum System: The utilization of a TMP in conjunction with reduction in spatial volume under vacuum, due to the elimination of the traditional large viewing chamber, yields a highly efficient and robust vacuum system for standard imaging as well as in-situ type analyses. This design also minimizes the potential for specimen damage and contamination allowing users to address a wide variety of specimen types I preparations.

High Precision Stage: The 5-axis ultra-stable goniometer with a 'ball-in-cup ' holder design for two steady points of conta_ct during specimen manipulation. This stage and holder design translates to increased image stability under a wide range of operating conditions. This is especially important during tilting / tomography and in high-throughput scenarios where high magnification imaging is necessary immediately after insertion of the holder into the electron column.

Sample Pre-Irradiation: Automated Pre-Irradiation (API) acclimates the specimen to the electron beam, reducing thermal expansion and helping to eliminate sample drift. This leads to an increase in sample stability and allows users to acquire high quality imaging data from less than ideal sample preparations.

20kV to 120kV Voltage Range: Low voltage range combined with the HC capability of the dual-mode lens, improves imaging of "unstained" and low contrast specimens. Reducing the need to stain specific samples saves time and improves environmental/ laboratory safety.

Simple Start-up and Shut-down: Activation of the accelerating voltage, filament voltage, and bias is computer controlled for quick and easy start-up. Users of various experience levels can effectively operate the microscope from start-up to shut-down with single button automated procedures.

Advanced Stage Navigation: The stage can trace movements, record positions / tilt angles, and utilize a low magnification navigation image so as to minimize repeated viewing of the same area or repeatedly exposing sensitive specimens to the electron beam. The operator can quickly verify that all areas of the specimen grid have been examined and easily recall saved positions for further analysis.

Reduced Specimen Damage: The TEM incorporates an air leak mechanism for the specimen pre-pumping chamber to prevent damage to sensitive samples during specimen exchange.

No Image Rotation: The image is stable (no rotation) throughout the entire magnification range.

Camera pixel size: Pixel size no larger than 6um (microns) x 6 um

Anticontamination device: A 'cold finger' or some similar anti-contamination device is useful to stabilize the sample and keep the microscope column clean.

Training: Training in the operation of the microscope and image acquisition software is essential.