Project Grant R61AI187113

Award Date 12/20/24
Completion Date 11/30/27
Dollars Obligated $345K
Federal Grant Program
93.855
Assistance Type
Project Grant
Place of Performance
Cincinnati, OH 45221, USA
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This federal Project Grant award from the National Institute of Allergy and Infectious Diseases (NIAID), under the Allergy and Infectious Diseases Research program (CFDA 93.855), provides $344,534 to establish ex vivo growth systems for the fungal genus Pneumocystis. The grant aims to:

  1. Conduct metabolite profiling and flux balance analysis to identify critical nutrients for growth of the Pneumocystis carinii species in a cell-free culture system. This work will leverage the surrogate rat model of Pneumocystis pneumonia.

  2. Develop alveolar organoids using rat lung cells to support the life cycle and growth of P. carinii.

The optimized cell-free medium and rat alveolar organoids will then be adapted to enable ex vivo growth of the human-specific Pneumocystis jirovecii species. This comprehensive, multidisciplinary approach encompasses metabolomics, computational modeling, and organoid cultures to overcome the key impediment of lacking an ex vivo growth system for these host-obligate fungi. The award period runs from December 2024 to November 2027.

The project also includes a $134,534 sub-award to Cincinnati Children's Hospital Medical Center to support the research.

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