This Project Grant award, funded by the National Center for Advancing Translational Sciences (NCATS) under CFDA Program 93.350, aims to establish and develop a high-throughput screening (HTS) assay to identify novel bacterial DNA gyrase poisoning inhibitors. The $298,045 grant, awarded to Top Biosciences, LLC on July 1, 2024, will support research focused on 1) developing the unique HTS assay, 2) utilizing T5 exonuclease and supercoiling-dependent fluorescence quenching (SDFQ) to confirm the identification of new gyrase poisoning inhibitors, and 3) miniaturizing and automating the HTS assay. The successful execution of this Phase I research could lead to the commercialization of a suite of biochemical HTS assays to screen large compound libraries for DNA topoisomerase inhibitors, including gyrase, which could aid in the development of new antibiotics to address the growing threat of antibiotic resistance.
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