This Project Grant award from the National Institute of General Medical Sciences (NIGMS) Biomedical Research and Research Training program (CFDA 93.859) is supporting the development of a 2-photon (2P) microscopy version of the SPOTLIGHT cell isolation technology. The goal is to create a versatile platform that can isolate individual cells from 3D samples after spatial and temporal profiling. In Phase I, the recipient, Imagen Bioworks Inc., will develop the necessary reagents and a 2P-SPOTLIGHT assay to enable the optical labeling and isolation of spatially distinct subpopulations of tumor-infiltrating T-cells within 3D cultures. This $333,048 award, with a period of performance from September 2024 to August 2025, aims to address the unmet need to isolate cells from 3D cultures for downstream spatiotemporal omics analysis, which is important for understanding disease mechanisms and identifying new drug targets.
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