Project Grant R35GM165884
- The National Institute of General Medical Sciences, part of the National Institutes of Health, awarded $345,581 to the University of Pennsylvania on September 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to visualize and characterize how bacteria and archaea use DNA-bending proteins to fold genomic DNA into three-dimensional shapes that regulate CRISPR-associated integrase function during adaptive immunity to phage infection. The project will employ...
- The National Institute of General Medical Sciences awarded $151,352 to the University of Pennsylvania on February 20, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to conduct structural and biochemical characterization of ribosomal RNA methylation by radical SAM enzymes. The project addresses antibiotic resistance mechanisms by examining how two radical S-adenosylmethionine (SAM) enzymes—CFR and RLMN—methylate adenosine residues in bacterial ribosomal RNA. CFR...
- The National Institute of General Medical Sciences awarded Health Research, Inc. Wadsworth Center $386,003 on August 1, 2026, to fund research on mechanisms of bacterial defense against conjugative plasmids and their phages under the Biomedical Research and Research Training program (CFDA 93.859). The research addresses antibiotic resistance by investigating how bacteria defend against conjugative plasmids—which spread resistance genes across bacterial populations—and plasmid-dependent phages...
- The National Institute of General Medical Sciences awarded the University of Pennsylvania $406,250 on March 2, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to advance nucleic acid chemistry and synthetic biology for RNA therapeutics. The research program develops de novo xeno nucleic acids (XNAs)—synthetic nucleic acid analogs with modified backbones that enhance nuclease resistance and extend half-life—to expand the genetic toolkit for RNA silencing and...
- The National Institute of Allergy and Infectious Diseases awarded $138,903 to the University of California, San Diego on January 17, 2026, under the Allergy and Infectious Diseases Research program (CFDA 93.855) to identify and characterize novel transcriptional regulators associated with antiphage immune systems in clinically important bacterial pathogens. The funded research focuses on gene regulation within bacterial defense islands. The recipient will define the upstream signals and...
- The National Institute of General Medical Sciences awarded Massachusetts Institute of Technology $127,567 on September 3, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to investigate mechanisms of nuclease-based bacterial immunity systems. The research, carried out in Cambridge, Massachusetts, through August 31, 2028, combines genetic and molecular biology approaches with training in biochemistry, structural biology, and computational methods to dissect how...
- The National Institute of General Medical Sciences awarded The Trustees of the University of Pennsylvania $438,609 on May 7, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to fund research combining gene editing with traction force microscopy to understand mechanotransduction in leukocyte motility. The research uses CRISPR-Cas9 gene editing and traction force microscopy to conduct a directed screen of molecules implicated in cell migration, specifically...
- The National Institute of General Medical Sciences awarded The Regents of the University of California, San Francisco $123,147 on May 13, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to investigate jumbo bacteriophage infection mechanisms and their potential as therapeutics against antibiotic-resistant bacteria. The research, conducted under the K99/R00 pathway, examines how FKZ-like jumbophages with genomes exceeding 200 kilobases evade bacterial immune...
- The National Institute of General Medical Sciences awarded the University of Pennsylvania $441,826 on September 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to investigate the molecular basis of transcription initiation at eukaryotic promoters in chromatin. The research will isolate and dissect pre-initiation complex (PIC)-mediator-coactivator complexes representing states from initiation to early elongation, using biochemical and structural approaches...
- The National Institute of General Medical Sciences awarded the University of Pennsylvania $924,095 on April 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to investigate molecular mechanisms controlling actin cytoskeleton dynamics. The research addresses four major knowledge gaps: the ARP2/3 complex's role in actin filament branching and regulation by nucleotide state and binding proteins; filament dynamics at barbed and pointed ends, including cooperative...
The National Institute of General Medical Sciences awarded The Trustees of The University of Pennsylvania $363,875 on September 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to investigate molecular mechanisms by which bacteriophages evade bacterial CRISPR-CAS immune systems. The research focuses on phage-encoded anti-CRISPR proteins, specifically ACRVA2, which inhibits the CAS12A nuclease through translation-dependent mRNA degradation. The investigator previously identified ACRVA2 as binding nascent CAS12A polypeptides emerging from ribosomes and triggering mRNA destruction before translation completion, enabling suppression of CAS12A variants despite nucleotide-level differences. Future work will characterize how ACRVA2 recognizes the nascent polypeptide and degrades its mRNA, and will explore over 1,800 ACRVA2 homologs identified on plasmids and phages infecting diverse bacterial species. The research will determine whether these homologs employ similar translation-dependent regulatory mechanisms to downregulate other bacterial genes beyond CRISPR evasion, potentially uncovering widespread gene-regulation strategies shaped by mobile genetic elements. Performance occurs in Philadelphia, Pennsylvania. The award runs through August 31, 2031. This is an investigator-initiated project grant supporting fundamental discovery research in basic biological processes underlying phage-bacteria interactions and antibiotic resistance mechanisms.
Mod # | Description | ReasonForModification | Federal Obligation | Date |
|---|---|---|---|---|
| Not listed | $363.9k | 8/6/26 |