Project Grant R35GM165704
- The National Science Foundation Division of Chemistry awarded the University of Washington $479,403 on August 15, 2026, under the Mathematical and Physical Sciences program (CFDA 47.049) to develop and optimize mass spectrometry methods for characterizing complex glycoproteins and their heterogeneous glycosylation states. The project will advance ion-ion reactions and tandem mass spectrometry instrumentation through combinations of collision-, electron-, and photon-based gas-phase activation,...
- The National Institute of General Medical Sciences awarded the University of Washington $2,226,244 on September 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to develop nanopore technology for single-molecule protein sequencing of intact proteoforms. The funded work advances unfoldase-coupled nanopore arrays to directly sequence native proteins at single-molecule resolution, detect post-translational modifications, and analyze complex proteoforms. The project...
- The National Institute of General Medical Sciences awarded the University of Washington $2,102,360 on May 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to develop ultrahigh multiplexing fluorescent probes and methods for spatial transcriptomics of tissue samples. The funded work encompasses design, synthesis, and characterization of over 1,000 spectrally and DNA-barcoded fluorescent probes conjugated with DNA oligonucleotide barcodes to enable single-molecule...
- Federal Grant Award Summary The National Institute of General Medical Sciences (NIGMS) awarded a $427,625 Project Grant to the University of Washington, effective September 1, 2025, through August 31, 2030, under the Biomedical Research and Research Training program (CFDA 93.859). This award supports the development of capillaric microfluidics technology for integrated, self-powered, and self-regulated sample preparation in miniaturized mass spectrometry (MS) systems. The research addresses a...
- The National Institute of General Medical Sciences awarded the University of Washington $1,618,487 on August 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to develop and demonstrate nanopore tweezers, a novel single-molecule technique for observing enzyme motion along nucleic acids with sub-nucleotide precision and sub-millisecond time resolution. The award funds development of nanopore tweezers technology to enable broader adoption by the scientific and...
- The National Institute of General Medical Sciences awarded the University of Washington $427,625 on July 5, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to investigate the molecular mechanisms by which membrane-tethered E3 ubiquitin ligases regulate cell-surface receptor abundance and signaling sensitivity. The research addresses how receiving cells dynamically fine-tune their sensitivity to extracellular signals, focusing on a poorly understood class of...
- The National Institute of General Medical Sciences awarded the University of Washington $397,158 on April 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to conduct research on the biosynthesis, discovery, and biocatalytic production of halogenated nucleosides and nucleoside-sulfamates. The research will investigate biosynthesis pathways of natural product nucleoside analogs containing rare functional groups, with emphasis on identifying key enzymes involved in...
- The National Institute of General Medical Sciences, National Institutes of Health, awarded the University of Washington $450,950 on August 5, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to characterize metastable thiolate intermediates in cysteinate-ligated metalloenzymes through small-molecule analogues and spectroscopic methods. The project will use electron paramagnetic resonance, Mössbauer, electronic absorption, X-ray absorption, and vibrational...
- The National Institute of General Medical Sciences awarded the University of Washington $1,368,400 on June 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to develop analytical tools for monitoring proline cis/trans isomerization in proteins. The recipient will develop tandem proteolysis combining cold liquid chromatography, proline-specific proteases, ion mobility mass spectrometry, and ultraviolet photodissociation to directly identify proline cis/trans...
- The National Science Foundation Division of Chemistry awarded the University of Washington $588,303 on August 15, 2026, under the Mathematical and Physical Sciences program (CFDA 47.049) to develop computational methods and experimental techniques for ion mobility–mass spectrometry that advance the measurement of protein and molecular structures and dynamics. The research group will create fast computational methods for interpreting ion mobility–mass spectrometry measurements and introduce a new...
The National Institute of General Medical Sciences awarded the University of Washington $232,520 on September 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to develop mass spectrometry and chemical glycoproteomics technologies for studying the glycocode—the collection of glycan-protein combinations that direct biological processes at the cell surface. The award funds development of a suite of new analytical tools to address three core challenges in glycoscience: improving sensitivity in glycoproteomics, establishing reproducibility and robustness in measurement protocols, and connecting glycocode regulation to cellular function. The recipient will expand autonomous dissociation-type selection (ADS) methodology to capture multiple classes of N- and O-glycosylation; explore liquid- and gas-phase separation strategies to increase sampling depth and disambiguate co-isolated glycopeptide precursors; and develop enrichment strategies using inactive point mutants of glyco-enzymes to target often-missed glycoproteins. The team will couple automated proteolytic digestion protocols with novel glycopeptide enrichment approaches using magnetic beads, then explore method parameters including compatible detergents, trypsin incubation conditions, enrichment buffers, and related variables to establish reproducible workflows. Place of performance is Seattle, Washington. The award runs through August 31, 2031. The University of Washington Office of Sponsored Programs administers the grant as the recipient organization.
Mod # | Description | ReasonForModification | Federal Obligation | Date |
|---|---|---|---|---|
| Not listed | $232.5k | 8/13/26 |