This $584,420 Project Grant, awarded by the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training program (CFDA 93.859), supports research into spatiotemporal mapping and engineering of the "dark proteome" - intrinsically disordered proteins and their functions. The principal objectives of the grant are to: (1) investigate the role of protein disorder in facilitating transport through the nuclear pore complex, including developing...
This federal Project Grant award from the National Institute of General Medical Sciences (NIGMS) Biomedical Research and Research Training Program (CFDA 93.859) provides $118,904 to The Regents of the University of California, San Francisco (UCSF) to support research aimed at revealing signal transduction mechanisms through protein design. The key products and services to be delivered under this 2-year award include: 1) Using protein design to generate minimal, tunable versions of modular...
This Project Grant award from the National Institute of General Medical Sciences (NIGMS), under the Biomedical Research and Research Training (CFDA 93.859) federal grant program, provides $360,100 to The Regents of the University of California, San Francisco (UCSF) to design and characterize novel photoacid-binding proteins for studying proton dynamics in biological systems. The key objectives are to: 1) use computational protein design tools to position photoacid cofactors within customized...
This $402,500 Project Grant award from the National Institute of General Medical Sciences (NIGMS) Biomedical Research and Research Training Program (CFDA 93.859) aims to develop a novel approach that combines modular fluorophores and correlative super-resolution microscopy to investigate the structure, dynamics, and interactome of biomolecular condensates in bacteria. The project, being conducted at New York University, will apply this "opto-proteomic toolbox" to understand the role of...
This Project Grant award from the National Institute of General Medical Sciences (NIGMS), under the Biomedical Research and Research Training program (CFDA 93.859), supports the development of engineered enzymes that can be activated to perform targeted modifications of cellular proteins. The $1,602,000 award to the Dana-Farber Cancer Institute, Inc. (DFCI) will fund research to design and apply novel autoinhibited enzymes that can toggle between inactive and active conformations in response...
This Project Grant award from the National Science Foundation (NSF) under the Biological Sciences program (CFDA 47.074) provides $369,297 to San Francisco State University (SFSU) to develop a synthetic transhydrogenation module to enhance the production of valuable chemicals using engineered organisms. The key objectives are to: Elucidate the structural mechanisms underlying the autoregulation of the GltAB-GudB enzyme complex in Bacillus subtilis, which regulates glutamate metabolism and impacts...
The National Institute of General Medical Sciences (NIGMS) awarded a Project Grant totaling $398,833 to The Trustees of the Smith College, doing business as Smith College, to expand the design space of protein-small molecule conjugates for targeted therapy and drug delivery applications. Specifically, the project aims to validate scaffold proteins and side-chain reactive polymers as modular components for novel protein-drug conjugate structures. The work will focus on modifying model FN3...
This Project Grant award from the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training Program (CFDA 93.859) provides $323,941 to The Trustees of Columbia University in the City of New York to elucidate how protein sequence influences the conformational dynamics that drive substrate transport by ATP-binding cassette (ABC) transporters. The research will leverage single-molecule fluorescence resonance energy transfer (SMFRET) techniques to...
This National Science Foundation Project Grant of $921,054 supports research to develop new biological functions through engineering plant hormone receptors. Led by the Regents of the University of California, Riverside under the Biological Sciences program (CFDA 47.074), the project engineers plant sensors to recognize pharmaceuticals and dietary molecules. It then uses these sensors to design "sentinel cells" that indicate molecule presence through color changes. Specifically, the...
This $400,000 Project Grant award from the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training program (CFDA 93.859) will fund research to develop an integrated computational framework for predicting and designing "photoenzymes" - enzymes that can utilize light to catalyze non-natural chemical reactions important for pharmaceutical synthesis. The key products or services to be delivered include: 1) Machine learning-enhanced...