This $479,924 five-year Project Grant awarded by the National Institute of General Medical Sciences (NIGMS) Biomedical Research and Research Training program (CFDA 93.859) supports fundamental research at The Pennsylvania State University, doing business as Penn State, to investigate how RNA structure regulates gene expression.
The research program aims to determine the mechanisms by which RNA polymerase pausing, transcription termination, and RNA-binding proteins like CsrA control gene expression co-transcriptionally in bacteria. Key approaches include comparative studies in E. coli and B. subtilis, genome-wide transcriptome profiling, RNA structural probing, metabolomics, and cryo-EM structural analysis. The findings are expected to uncover new RNA-based regulatory mechanisms responding to cellular stresses and metabolites. In addition, the project will develop new experimental methods that can be broadly applied to study RNA regulation in diverse organisms.
Generated 3/4/25, 7:21 AM