Project Grant R24OD038113
- The National Science Foundation (NSF) awarded a $299,772 EAGER (Early-concept Grants for Exploratory Research) grant under the Biological Sciences program (CFDA 47.074) to Washington State University (WSU) to develop new molecular tools for identifying protein-protein interactions within specific cellular compartments in plant cells. The project, titled "TOOLS4CELLS: Bimolecular Proximity Tagging", aims to close the gap in existing methods by creating split enzyme versions that can map...
- The National Science Foundation awarded $670,036 to the University of California, San Diego under Project Grant number 47.041, the Engineering program, to support the project "ELUCIDATING ESSENTIAL FACTORS FOR PRODUCTION OF RECOMBINANT SECRETED PROTEINS THROUGH PROTEOMICS" from April 1, 2021 through March 31, 2024. The University of California, San Diego will utilize the funding to elucidate essential factors for the production of recombinant secreted proteins through proteomics. As...
- This $837,754 National Science Foundation project grant will fund research into the impact of the KFERQ protein degradation motif in the roundworm Caenorhabditis elegans. A collaborative team from the City University of New York's Queens College and an Israeli institution will use genetically engineered C. elegans expressing a fluorescent fusion protein to study how proteins are selected and broken down based on the KFERQ motif sequence. The researchers aim to elucidate the molecular...
- This $350,000 project grant awarded by the National Science Foundation (NSF) Engineering program (CFDA 47.041) aims to develop a yeast strain with a robust protein secretion system that is tolerant to extreme pH and temperature levels. The primary objectives are to engineer the secretory pathway of the yeast Kluyveromyces marxianus using gene editing tools such as CRISPR, identify critical genes influencing protein production and secretion, and develop effective CRISPR libraries and selection...
- This four-year, $822,736 project grant from the National Science Foundation's Division of Integrative Organismal Systems and Biological Sciences program will fund research into the role of extracellular RNA in intercellular and interkingdom communication. Led by Clemson University, the collaborative project brings together seven laboratories to advance understanding of how RNA secreted by host cells maintains healthy microbiomes in plants, insects, and humans. The researchers will investigate...
- Project Grant Award Summary The University of California Santa Cruz received a $763,602 Project Grant from the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training program (CFDA 93.859), effective August 1, 2025, through May 31, 2030. This research initiative, titled "Mechanisms of Nematode Molting," will deliver fundamental scientific research aimed at understanding how biological developmental timers control animal development and...
- Partillion Bioscience Corp. was awarded a $659,037 Project Grant from the National Institutes of Health's National Institute of General Medical Sciences to develop reagent products enabling high-throughput screening and selection of single cells based on secreted bio-products. Under the Biomedical Research and Research Training program (CFDA 93.859), Partillion plans to transition its lab-on-a-particle technology—highlighted as a 2020 SLAS Innovation Award winner—into the first reagent product...
- Federal Grant Award Summary Epigenter, LLC received a $298,530 Project Grant from the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training program (CFDA 93.859) for the period July 1, 2025 through June 30, 2027. The award supports the development of epigenetic editor therapeutics—a novel therapeutic approach that modulates cytosine methylation patterns in gene promoter regions without altering the patient's DNA sequence. The research aims...
- Federal Grant Award Summary Cornell University's Office of Sponsored Programs received a $375,154 Project Grant award from the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training program (CFDA 93.859), effective June 1, 2026, through February 28, 2031. The award supports a five-year research initiative to uncover the structure and function of understudied membrane proteins implicated in human diseases. Using Caenorhabditis elegans as a model...
- Summary Active Motif, Inc. received a $306,094 Project Grant award from the National Human Genome Research Institute (NHGRI) under the Human Genome Research program (CFDA 93.172), with performance extending from September 20, 2025, through August 31, 2026. The award funds the development of a novel epigenetic editing platform that utilizes an innovative targeting approach to recruit epigenetic effectors to specific genomic loci. This platform enables targeted alterations to the epigenome and...
ILLUMINATING THE SECRETOME: A PROTEIN TAGGING RESOURCE - SUMMARY ABERRANT EXPRESSION AND SECRETION OF PROTEINS IN THE SECRETOME HAVE BEEN LINKED TO NUMEROUS DISEASES. NEMATODE SECRETOMES ARE OF ADDITIONAL SIGNIFICANCE AS THEY MEDIATE INTERACTIONS WITH HOST TISSUES AND PRESENT OPPORTUNITIES TO DISCOVER NOVEL IMMUNOMODULATORY COMPOUNDS. ALTHOUGH SECRETOME PROTEINS ARE ENRICHED FOR DRUG TARGETS AND BIOMARKERS FOR DISEASE AND AGING, THE GAP IN KNOWLEDGE CONCERNING THE IN VIVO DISTRIBUTION OF SECRETED PROTEINS IS A BARRIER TO LEVERAGING AND CONTEXTUALIZING INFORMATION WE HAVE SO FAR TO FULLY UNDERSTAND THEIR BIOLOGICAL FUNCTION. TO ADDRESS THIS GAP, WE WILL CREATE A RESOURCE OF GENETIC STOCKS EXPRESSING ENDOGENOUSLY TAGGED SECRETOME PROTEINS USING A HIGHLY TRACTABLE MODEL ORGANISM, THE NEMATODE C. ELEGANS. WE AND OTHERS HAVE ESTABLISHED THE FEASIBILITY AND UTILITY OF LARGE-SCALE TAGGING OF SECRETED EXTRACELLULAR MATRIX PROTEINS BY FLUORESCENT PROTEIN AND AFFINITY TAGS. A HALLMARK OF OUR APPROACH IS THE USE OF 'KNOCK-IN' EDITING OF ENDOGENOUS GENES, TO AVOID MIS/OVER-EXPRESSION AND TO ALLOW GENERATION OF STABLE GENETIC STRAINS THAT ALLOW REPRODUCIBLE AND COMPARABLE ANALYSIS OF EXPRESSION PATTERNS. WE WILL PRIORITIZE 1000 HIGH-VALUE GENES OF WIDEST INTEREST TO THE BIOMEDICAL COMMUNITY. THIS TARGET SET WILL INCLUDE 410 SECRETOME PROTEINS WITH ORTHOLOGS IMPLICATED IN HUMAN DISEASES, AGING, OR PROTEOSTASIS; 320 PROTEINS PROMINENT IN EXCRETORY-SECRETORY PRODUCT (ESP) PROTEOMICS OR INNATE IMMUNE ROLES; AND 270 REPRESENTATIVES OF CONSERVED SECRETOME PROTEIN FAMILIES AND PROTEINS SECRETED BY UNCONVENTIONAL SECRETORY PATHWAYS. WE HAVE OUR INITIAL LIST OF 1000 TARGETS AND WILL PRIORITIZE SELECT TARGETS BASED ON INPUT FROM THE C. ELEGANS AND BROADER SCIENTIFIC COMMUNITY. SECRETOME GENES WILL ALSO BE CURATED USING STRUCTURE-BASED TOOLS TO CLASSIFY PROTEINS CURRENTLY UN-ANNOTATED BASED ON PRIMARY SEQUENCE. GENETICALLY TAGGED, STABLE KNOCK-IN STOCKS WILL BE DEPOSITED IN THE STOCK CENTER (CGC) FOR DISTRIBUTION AS SOON AS THEY ARE VALIDATED, AND MOLECULAR OR EXPRESSION INFORMATION MADE AVAILABLE ON CGC AND WORMBASE DATABASES AS WELL AS A UCSC-HOSTED WEBSITE. IN ADDITION TO THE OVERARCHING GOAL OF GENERATING VALIDATED AND CURATED KNOCK-IN STRAINS, RELATED TECHNICAL AND SCIENTIFIC AIMS WILL BE PURSUED, AND GENE EDITING TECHNOLOGY WILL BE FURTHER OPTIMIZED TO ENHANCE THE VALUE OF THE RESOURCE. AS THE SECRETOME IS ENRICHED IN SMALL PROTEINS (
Mod # | Description | ReasonForModification | Federal Obligation | Date |
|---|---|---|---|---|
| Not listed | $790.5k | 6/8/26 |