This Project Grant award, valued at $149,101, was provided by the National Institute of Biomedical Imaging and Bioengineering under the Discovery and Applied Research for Technological Innovations to Improve Human Health (CFDA 93.286) federal grant program. The award supports the development of a compact, chip-scale flow-based single-molecule assay (SEMI-SIMOA) system that integrates electronics and microfluidics onto a single integrated circuit chip. This system aims to electronically count individual sandwiched immunocomplexes as they pass through on-chip pores, enabling the detection of low-abundance protein biomarkers related to neurodegenerative diseases, such as alpha-synuclein, neurofilament light, and glial fibrillary acidic protein. The project objectives focus on implementing the CMOS-embedded microfluidics, sensing pores, and readout circuits, as well as functionalizing nanoparticles with target-specific antibodies and validating the assay performance. The successful completion of this project could transform future diagnostics for diseases requiring the detection of low-abundance biomarkers, particularly in resource-limited settings.
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