The National Institute of Biomedical Imaging and Bioengineering (NIBIB) awarded a $389,751 Project Grant under CFDA 93.286, "Discovery and Applied Research for Technological Innovations to Improve Human Health," to the Regents of the University of Minnesota, Office of Sponsored Projects Administration. This 2-year grant aims to develop a low-cost, compact holographic imaging-based microfluidic cell sorting system using 3D printing. The research will explore the use of 3D holographic imaging and multi-material 3D printing techniques to overcome the depth of field limitations of conventional microscopic imaging, enabling high throughput and precise cell sorting for critical clinical applications such as rare cell detection and liquid biopsy for cancer diagnostics. The 3D printing approach is expected to reduce fabrication costs and time while improving the reliability and imaging quality of the microfluidic devices. This project has the potential to enable new designs of high-performance, versatile microfluidic systems for various medical research and clinical use cases.