Project Grant R03AI180614
- This $780,162 Project Grant from the National Institutes of Health's National Institute of Allergy and Infectious Diseases will fund research at Old Dominion University from August 2022 through July 2026 exploring the role of nutrient and stress sensing in regulating Clostridioides difficile physiology and behavior within the host. The research aims to determine how the stringent response regulates disease-relevant bacterial processes and characterize enzymes that mediate alarmone metabolism...
- This Project Grant, awarded on January 1, 2026, by the National Institute of Allergy and Infectious Diseases (NIAID) under the Allergy and Infectious Diseases Research program (CFDA 93.855), provides $151,040 in funding to Tufts University School of Medicine to conduct fundamental research on bacterial cell division mechanisms in Clostridioides difficile. The research will identify and characterize the unique divisome—the multiprotein complex responsible for bacterial cell division—in this major...
- The National Institutes of Health (NIH) has awarded a $1,333,682 Project Grant under the Trans-NIH Research Support program (CFDA 93.310) to North Carolina State University (NC State) to develop peptide inhibitors that neutralize bacterial toxins and deliver them using engineered probiotic yeast. This 3-year project aims to expand on NC State's previous work identifying a lead peptide inhibitor of Clostridioides difficile toxin, by screening for inhibitors of 5 additional bacterial toxins. The...
- Duke University's Office of Research Administration received a $184,651 Project Grant from the National Institute of Allergy and Infectious Diseases (NIAID) under the Allergy and Infectious Diseases Research program (CFDA 93.855) awarded on August 8, 2025, with completion expected by July 31, 2030. The research initiative focuses on understanding the progression from Clostridioides difficile colonization to active disease, addressing a significant public health challenge with over 450,000...
- Federal Grant Award Summary Tufts University School of Medicine received a $136,104 Project Grant award dated October 24, 2025, from the National Institute of Allergy and Infectious Diseases (NIAID) under the Allergy and Infectious Diseases Research program (CFDA 93.855). The grant funds investigator-initiated research examining how the spatial distribution and epithelial association of Clostridioides difficile bacteria, combined with heterogeneous toxin gene expression, influence disease...
- Federal Grant Award Summary The National Institute of General Medical Sciences (NIGMS) awarded $532,500 through the Biomedical Research and Research Training program (CFDA 93.859) to University Enterprises, Inc. (doing business as Sacramento State Sponsored Research) for a three-year project spanning September 1, 2025, through August 31, 2028. The project investigates the function and evolution of bacterial strains within the human gut microbiota, specifically focusing on Paraclostridium...
- This three-year National Science Foundation project grant of $240,000 supports research investigating how bacterial endospore formation affects host-microbe codiversification. The grantee will analyze metagenome-assembled genomes from museum rodent fecal samples to test for congruence between bacterial and rodent host phylogenies, indicating long-term associations. Bacteria will also be cultured from feces under anaerobic conditions and assayed for endospore formation. Phylogenetic multi-level...
- Federal Grant Award Summary Texas A&M University System Health Science Center received a $161,859 Project Grant award from the National Institute of Allergy and Infectious Diseases (NIAID) under the Allergy and Infectious Diseases Research program (CFDA 93.855) effective August 11, 2025, with a completion date of July 31, 2027. The grant supports research to identify novel antivirulence drug targets for Clostridioides difficile infection (CDI), an antibiotic-associated condition that...
- This Project Grant award from the National Science Foundation's Biological Sciences program (CFDA 47.074) provides $788,966 to Boston College to investigate how microbial community interactions influence the success or failure of new microbes invading existing communities. Through a combination of mathematical modeling and experimental validation using laboratory communities of nasal bacteria, the project aims to elucidate three key questions: (1) how nutrient availability impacts invasion...
- The National Science Foundation Division of Molecular and Cellular Biosciences awarded a $668,913 Project Grant to the University of Massachusetts Office of Grant & Contract Administration to support research titled "Collaborative Research: Model-Guided Design of Bacterial Interspecies Interactions and Trans-Organismic Communication in Living Intercellular Circuits." The award aims to advance understanding of interspecies biochemical cell-cell communication in natural microbial...
CONTACT-DEPENDENT SUPPRESSION OF CLOSTRIDIOIDES DIFFICILE SPORULATION BY ENTEROCOCCI - SPORULATION BY CLOSTRIDIOIDES DIFFICILE OCCURS NATURALLY IN THE GUT AMONG OTHER BACTERIA SUCH AS ENTEROCOCCI, WHICH ARE PRESENT IN ALL ANIMALS. THE LONG-TERM GOAL OF THIS LABORATORY IS TO DEFINE THE MECHANISTIC BASIS OF BACTERIA-BACTERIA AND HOST INTERACTIONS THAT MODULATE VIRULENCE OF C. DIFFICILE. THE OVERALL OBJECTIVE OF THIS PROPOSAL IS TO DISCOVER GENETIC FACTORS INVOLVED IN A POTENTIAL PROXIMITY-DEPENDENT INHIBITION OF C. DIFFICILE SPORULATION BY ENTEROCOCCI. THE CENTRAL HYPOTHESIS IS THAT ENTEROCOCCI SUPPRESS C. DIFFICILE SPORULATION VIA INTERACTIONS AT THE CELL SURFACE. THE RATIONALE FOR THIS PROJECT IS THAT DETERMINATION OF MECHANISMS OF INTERACTION BETWEEN THE TWO SPECIES WILL ALLOW THE DEVELOPMENT OF TREATMENTS THAT LIMIT THE POTENTIAL FOR C. DIFFICILE INFECTION. BASED ON PRELIMINARY DATA, OUR OVERALL WORKING HYPOTHESIS FOR THIS PROPOSAL IS: E. FAECALIS INHIBITS SPORULATION VIA ITS SURFACE PROTEINS WHEN IN CELL-CELL CONTACT WITH C. DIFFICILE. TO ATTAIN THE OVERALL OBJECTIVES THESE TWO SPECIFIC AIMS WILL BE PURSUED: AIM 1) IDENTIFY GENES IN ENTEROCOCCUS FAECALIS OG1RF THAT ARE INVOLVED IN PROXIMITY-DEPENDENT INHIBITION OF C. DIFFICILE SPORULATION. IN THESE EXPERIMENTS, WE WILL USE AN ARRAYED LIBRARY OF TRANSPOSON INSERTION MUTANTS TO SCREEN FOR GENES IN E. FAECALIS INVOLVED IN SUPPRESSION OF SPORULATION. AIM 2) DEFINE THE TRANSCRIPTIONAL NETWORK OF ENTEROCOCCI-MEDIATED SPORULATION INHIBITION. WE WILL DEVELOP A WELL CALIBRATED TRANSWELL CO-CULTURE ASSAY AND WILL PERFORM TRANSCRIPTIONAL PROFILING DURING INDUCTION OF SPORULATION TO DETERMINE GENES INVOLVED IN THIS PHENOMENON. THE RESEARCH PROPOSED IS INNOVATIVE IN THE APPLICANT'S OPINION BECAUSE IT FOCUSES SPECIFICALLY ON INTERACTIONS BETWEEN TWO GASTROINTESTINAL PATHOGENS AND HOW CELL CONTACT WITH NON-KIN BACTERIA REGULATES A CELL FATE DECISION. THE PROPOSED RESEARCH IS SIGNIFICANT BECAUSE IT WILL PROVIDE A CONTEXT FOR UNDERSTANDING HOW ENTEROCOCCI, WHICH COMMONLY EXPAND IN ANTIBIOTICS-TREATED PATIENTS, AFFECT THE BALANCE BETWEEN SPORE FORMATION AND VEGETATIVE TOXIN-PRODUCING C. DIFFICILE.
Mod # | Description | ReasonForModification | Federal Obligation | Date |
|---|---|---|---|---|
| Not listed | $0 | 4/2/26 | ||
| Not listed | $0 | 3/30/26 | ||
| Not listed | $75.7k | 1/22/26 | ||
| Not listed | ($73k) | 1/22/26 | ||
| Not listed | $75.7k | 1/22/26 |