The National Institute of Environmental Health Sciences (NIEHS) awarded Emory University a 5-year, $654,756 Project Grant (CFDA 93.113 - Environmental Health) to conduct the OMICS and Mixtures Integration on Traffic Exposure and Preterm Birth (OMIT-PTB) study. This project aims to integrate longitudinal multi-omics data, spanning the maternal epigenome, metabolome, and microbiome, to characterize the molecular connections between maternal exposures to traffic-related air pollution (TRAP) and preterm birth (PTB) outcomes. The study will leverage the Atlanta African American Maternal-Child Cohort to conduct state-of-the-art multi-omics profiling and integration during early and late pregnancy, innovative mixture analyses, and advanced assessment of cumulative and short-term TRAP exposures on 700 pregnant individuals. The goal is to provide novel insights and comprehensive information on signatures of systemic biological perturbation linking TRAP exposures to PTB, leading to the identification of potential biomarkers and preclinical indicators for PTB. This research will contribute to elucidating mechanisms and risk factors for TRAP-associated adverse birth outcomes, informing strategies to mitigate exposure risks and improve maternal and child health in impacted communities.