The Project Grant award K99HG013991, totaling $126,830, was provided by the National Human Genome Research Institute (NHGRI) under the Human Genome Research (CFDA 93.172) Federal Grant Program. The award supports the development of a somatic mutation-resolved lineage tracing system that will reconstruct single-cell genealogical trees and detect associated somatic mutations and epigenetic alterations. This system will be used to study the acquisition histories of somatic mutations on hematopoietic stem cell (HSC) genealogical architecture, charting the evolutionary trajectory of normal aging hematopoiesis and leukemic transformation. The project also aims to develop a genealogy-resolved CRISPR screening approach to survey the causative effects of somatic modifications on cellular dynamics and functions. This research is anticipated to advance the understanding of genomic evolution in somatic cells and its impact on cellular behaviors and cell fate choices. The award was granted to The Children's Hospital Corporation, doing business as Boston Children's Hospital, a non-profit pediatric teaching hospital and research center affiliated with Harvard Medical School.
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