This $1,737,000 Project Grant awarded by the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training program (CFDA 93.859) supports the development of innovative single-cell genomics technologies for joint analysis of regulatory dynamics and transcriptional states. Key objectives include:
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Developing multiomics tools to measure rates of epigenomic changes (DNA methylation, demethylation, oxidative damage, DNA repair) and relate these to transcriptional states in single cells.
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Creating a technology to detect colocalized regulatory elements and their epigenetic states along with transcriptomes from individual cells, to assess regulatory functionality.
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Integrating a high-definition 3D spatial registration platform (AMBER) with the single-cell multiomics tools to reconstruct tissue-scale molecular profiles.
These new capabilities will be applied to investigate molecular changes associated with aging in the mouse nervous system, with the potential for broader applicability to studying regulatory dynamics in health and disease. The grant was awarded on September 1, 2023 with an expected completion date of August 31, 2026. A sub-award totaling $112,000 was provided to Weill Medical College of Cornell University to support specific aspects of the project.
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