This Project Grant award from the National Science Foundation (NSF) Biological Sciences program (CFDA 47.074) is aimed at developing new functional genomics tools for the filamentous cyanobacterium Nostoc punctiforme. The $701,884 project, awarded to The Regents of the University of Colorado, will create a toolkit including a random barcode transposon-site sequencing (RB-TnSeq) library, CRISPR-based gene editing, and proximity labeling to study protein interactomes. This will enable improved understanding of the genome-phenome relationship in filamentous cyanobacteria, which have important ecological roles and applications in biotechnology like biofuel and biofertilizer production. The project will also generate training materials to facilitate adoption of these tools by other research groups. Additionally, the award will support undergraduate and graduate student training, integration of the research into college courses, and an educational exhibition.
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