The National Science Foundation (NSF) awarded a $300,000 Project Grant under its Biological Sciences program (CFDA 47.074) to the Georgia TECH Research Corporation for the "TOOLS4CELLS: EAGER: A MOLECULAR PURSUIT FOR THE ENGRAM: MICROFLUIDIC TEMPORAL TRANSCRIPTOMICS FOR SINGLE CELL LEARNING" project. This 2-year effort aims to develop innovative microfluidic tools and workflows to investigate the role of non-coding RNA in learning and memory storage in single-celled organisms. Key...
This National Science Foundation (NSF) Project Grant under the CFDA program "Biological Sciences" (47.074) is awarded to Trustees of Boston University to develop and optimize optogenetic tools for selecting bacterial cells based on dynamic, time-varying traits. The $796,158 award, effective August 1, 2023 through July 31, 2026, supports the researchers in creating light-inducible genetic constructs that trigger antibiotic resistance, enabling the isolation of bacteria exhibiting...
This $573,937 five-year Project Grant was awarded on February 1, 2024 by the National Science Foundation's (NSF) Division of Molecular and Cellular Biosciences under the Biological Sciences program (CFDA 47.074). The grant will fund research at the Massachusetts Institute of Technology (MIT) to understand how changes in DNA structure impact the behavior of synthetic gene circuits and gene expression more broadly. The project aims to develop a better understanding of supercoiling-mediated...
This two-year, $211,846 project grant from the National Science Foundation's Division of Materials Research supports research into programmable living materials through synthetic biofilm engineering and quantitative computational modeling at the Massachusetts Institute of Technology. The researchers will develop bioengineering tools to control bacterial cell deposition and assembly into "material blocks" with distinct interfaces. They will also establish modeling approaches to...
This National Science Foundation project grant award of $600,000 supports research into the mechanisms underlying long-term memory storage at the University of California, Los Angeles from February 1, 2022 to January 31, 2025. The award is made under the Biological Sciences program (CFDA 47.074) to promote progress in the biological sciences. Specifically, the grant funds research investigating whether memories are stored through nuclear modifications in neurons rather than changes in...
The National Science Foundation awarded a $468,457 CAREER grant to Worcester Polytechnic Institute (WPI) under the Biological Sciences program (CFDA 47.074) to investigate the impact of epigenetic regulation on gene expression and synthetic gene circuit function in the soil bacterium Pseudomonas putida. The 5-year project aims to: 1) Determine how genome methylation affects endogenous gene expression of P. putida in soil, and 2) Control synthetic gene circuit expression in P. putida by...
This $650,000 federal Project Grant award from the National Science Foundation (NSF) Computer and Information Science and Engineering (CISE) program (CFDA 47.070) supports the development of a transformative on-surface data storage and computing platform based on programmable DNA molecules. The Massachusetts Institute of Technology (MIT) is leading this research to overcome limitations of conventional silicon-based computing and data storage technologies. The project aims to pattern DNA...
The National Science Foundation awarded a $367,636 Project Grant to the Massachusetts Institute of Technology from March 1, 2021 through February 29, 2024 under the Biological Sciences program (CFDA 47.074). The grant funds collaborative research on multidimensional single-cell phenotyping to elucidate relationships between genomes and phenotypes. The Biological Sciences program aims to advance biological knowledge and understanding of major problems through basic research. This award supports...
This $604,209 federal Project Grant award from the Office of Naval Research (ONR) under the Basic and Applied Scientific Research program (CFDA 12.300) supports fundamental and applied research by the Massachusetts Institute of Technology (MIT) in the area of VBFF ANALOG EPIGENETIC CELL MEMORY BIOLOGY AND ENGINEERING. The grant, which runs from December 1, 2024 through November 30, 2029, will enable MIT to conduct advanced scientific research and technology development across disciplines such as...
This Project Grant award from the National Science Foundation (NSF) Biological Sciences program (CFDA 47.074) provides $240,000 in funding to The Johns Hopkins University to explore the interactions between CRISPR-Cas immune systems and temperate bacteriophages. The project aims to understand how CRISPR-Cas systems, which protect bacteria from viral infections, can acquire memories about temperate phages that can enter a dormant "lysogenic" state within bacterial cells. The research...
This Project Grant award from the National Science Foundation (NSF) Division of Molecular and Cellular Biosciences under the Biological Sciences program (CFDA 47.074) provides $481,237 to the Massachusetts Institute of Technology (MIT) to develop reversible long-term memory devices in bacterial cells. The key products and services to be delivered include:
Demonstrating the engineering of a circuit motif in bacteria that mimics processes associated with long-term memory of chromatin states in mammalian cells, enabling long-term memory of gene states in bacteria. This research aims to establish the molecular mechanisms that control the temporal duration of memory in bacteria, and develop bacterial memory devices that can last several weeks before reversal. The project will also apply the memory device technology to a biocontainment test-bed. The research and development effort will train graduate students in mechanical, biological, and electrical engineering at MIT, as well as provide undergraduate research experience to MIT and non-MIT students.