This Project Grant from the National Science Foundation Division of Polar Programs, under the Polar Programs federal grant program (CFDA 47.078), provides $210,495 to support research examining how microbial extremophiles in Antarctica respond to environmental changes in salinity. Led by researchers at the University of Tennessee, the grant will fund comparative analyses of gene expression patterns and secondary metabolite production in lab microcosm studies using microbial isolates previously collected from hypersaline sites in Antarctica. Transcriptomes of cell cultures under varying salinities will be sequenced and compared to determine significant changes to cellular metabolism and secondary metabolic pathways. At least one graduate student and four undergraduates will be involved in the research, which seeks to further understanding of life in extreme Antarctic environments and help elucidate the genetic basis for adaptation to changing conditions. Broader impacts include strengthening STEM research and education opportunities for women and underrepresented groups.
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