This $2,841,262 federal Project Grant awarded by the National Science Foundation (NSF) Biological Sciences program (CFDA 47.074) supports research at Oregon State University (OSU) to leverage the natural diversity of Agrobacterium bacteria to enhance genetic transformation and regeneration capabilities in woody plant species, with a focus on the economically important poplar. The key objectives are to screen ~100 novel Agrobacterium strains, analyze the genetic factors underlying high...
The Department of Agriculture's National Institute of Food and Agriculture awarded a $649,916 Project Grant to Oregon State University under the Biotechnology Risk Assessment Research (BRAG) program (CFDA 10.219). The grant supports research to develop improved tools for excising transgenes used in genetic engineering, particularly for plants like forest trees that are difficult to genetically modify and have high ecological impacts. The research will: 1) create novel recombinase target sites...
This $699,870 Project Grant award from the National Science Foundation's (NSF) Biological Sciences program (CFDA 47.074) supports the development of simplified and highly efficient genetic modification technologies for economically important grasses and cereal crops. The "PLANTTRANSFORM: SMART GRASS" project aims to overcome the current constraint in the plant science research community regarding the capacity to genetically modify major crop plants. The award establishes a...
This $180,000 Project Grant was awarded by the U.S. Department of Agriculture (USDA) National Institute of Food and Agriculture (NIFA) under the Agriculture and Food Research Initiative (AFRI) program (CFDA 10.310). The overall goal of this 3-year project is to identify genes related to vein initiation and spacing in the leaves of corn. To achieve this, the project will use three approaches: a genome-wide association study to associate vein density measurements with genetic variation,...
This $650,000 Project Grant, awarded by the U.S. Department of Agriculture National Institute of Food and Agriculture (USDA-NIFA) under the Biotechnology Risk Assessment Research (BRAG) Program (CFDA 10.219), supports the development of tools and frameworks to assist in the threat assessment of genetically engineered microorganisms (GEMs) for agricultural applications. The key objectives of the "STRATAGEM" project are to develop a standardized suite of DNA watermarking and laboratory...
This $451,139 Project Grant award from the National Science Foundation (NSF) Biological Sciences (CFDA 47.074) program supports the development of a new biotechnology called Transposase-Assisted Homology-Independent Targeted Insertion (TAHITI) for intragenic genome engineering of plants. The research aims to improve crop traits like disease resistance and yield by precisely inserting DNA sequences sourced from the same plant species, rather than relying on transgenic approaches that introduce...
The Project Grant award provides $682,500 to Oregon State University (OSU) from the USDA National Institute of Food and Agriculture (NIFA) under the Agriculture and Food Research Initiative (AFRI) program (CFDA 10.310). The funding supports research to characterize the susceptibility of Douglas-fir and western hemlock trees to the invasive plant pathogen Phytophthora pluvialis, use phylogenetic analysis to understand pathogen populations, and develop a CRISPR-based detection assay. This work...
The Oregon State University was awarded a $642,301 Project Grant from the United States Department of Agriculture National Institute of Food and Agriculture under the Agriculture and Food Research Initiative (AFRI) federal grant program (CFDA #10.310). The grant will support the development of an integrated computational and molecular workflow for rapidly designing CRISPR-based diagnostic tools for plant diseases. Specific objectives include expanding an existing Python algorithm to design...
This $649,847 Project Grant was awarded by the U.S. Department of Agriculture's National Institute of Food and Agriculture (USDA NIFA) under the Biotechnology Risk Assessment Research Grants Program (CFDA 10.219). The grant funds research to understand how pests overcome plant resistance traits conferred by engineered genes, and develop a monitoring framework to improve the durability of transgenic crops. The research focuses on characterizing the role of gene copy number variants (CNVs) in...
This $314,560 Project Grant award from the National Science Foundation (NSF) Division of Emerging Frontiers under the Biological Sciences program (CFDA 47.074) supports collaborative research by Purdue University to develop crop varieties that enhance agricultural sustainability. The key objectives are to: Identify genetic targets underlying microbiome-mediated genetic resistance to major current and emergent biotic and abiotic stresses in three widely-grown crops - wheat, tomato, and poplar...
This $225,000 Project Grant awarded by the U.S. Department of Agriculture's National Institute of Food and Agriculture under the Agriculture and Food Research Initiative (CFDA 10.310) aims to engineer improved plant genetic engineering capabilities. The key objectives are to:
Develop a toolkit for making modifications to various Agrobacterium strains, including those commonly used and novel wild-type strains, to enhance the efficiency and applicability of plant genetic engineering processes.
Design a new system for rapidly and efficiently installing existing genetic tools that boost gene delivery rates from Agrobacterium to plants into the new engineered strains.
The project will produce several enhanced Agrobacterium strains as a proof-of-concept, then evaluate their gene delivery capabilities against conventional strains when used for genetic engineering of difficult plant species like hops and trees. The resulting genetic tools and resources will be made available to other researchers and crop developers to help improve engineering processes for many additional crops. The project will be conducted by Oregon State University over a 2-year period from July 2024 to July 2026.