The Department of Agriculture Agricultural Research Service awarded a $262,250 firm-fixed-price purchase order to Oxford Nanopore Technologies Inc., a foreign-owned for-profit organization, to provide a single-molecule long read sequencer. This contract is a total small business set-aside with a NAICS code of 334516 and 1,000-employee size standard. The sequencer must be capable of generating long and ultra-long DNA sequence reads, with a potential output of over 100 gigabases per flowcell and read quality of Q20 or higher. The contract requires 192 sequencing runs over eight scheduled reagent shipments, with delivery to the USDA Pacific Basin Agricultural Research Center in Hilo, Hawaii within 120 days of award. This award supports genomic research initiatives at the USDA.