P00001 THE PRIMARY FOCUS OF THE PLANETARY PROTECTION GROUPS AT NASA AND JET PROPULSION LABORATORY (JPL) IS THE BIOLOGICAL SAMPLING OF SPACECRAFT TO ENSURE INTERNATIONAL PLANETARY PROTECTION REQUIREMENTS ARE MET. SPACECRAFT UNDERGO RIGOROUS BIOBURDEN (MICROBIAL LOAD) REDUCTION MEASURES PRIOR TO LAUNCH TO MEET THESE LIMITS. DESPITE ATTEMPTS BY JPL AND NASA TO REDUCE THE BIOBURDEN USING VARIOUS CLEANING REAGENTS AND METHODS, MICROORGANISMS PERSIST ON THE SPACECRAFT. MOREOVER, ALTHOUGH STUDIES ARE CONSTANTLY EXPANDING OUR UNDERSTANDING OF LIFE IN EXTREME ENVIRONMENTS, IT IS STILL UNCLEAR WHETHER MICROORGANISMS FROM EARTH, TRAVELING ON SPACECRAFT, CAN SURVIVE THE EXTREME ENVIRONMENTS THAT EXIST ON OTHER PLANETARY BODIES. THIS INFORMATION IS OF PARAMOUNT IMPORTANCE TO THE CURRENT MARS 2020 MISSION TO SEEK LIFE OUTSIDE OF EARTH. OVER THE DECADES NASA HAS IMPLEMENTED A NUMBER OF DIFFERENT CLEANING METHODS BUT VALIDATION STUDIES ARE OFTEN LACKING AND CURRENT NASA BIOBURDEN REDUCTION PROCEDURES DO NOT YIELD THE BIOBURDEN LEVELS MANDATED FOR SPACECRAFT TO EXPLORE AREAS OF MARS DEEMED AS "SPECIAL REGIONS" WHERE LIFE IS MOST LIKELY TO EXIST. THE RESEARCH PROPOSED FOR THIS PROJECT AIMS TO 1.) ASSESS SURVIVABILITY OF MICROORGANISMS COLLECTED FROM MARS 2020 TO SIMULATED MARS CONDITIONS TO DETERMINE WHICH MICROORGANISMS ARE THE HARDIEST TO EXTREME PLANETARY CONDITIONS ON MARS AND OF MOST CONCERN TO PLANETARY PROTECTION; AND 2.) CHARACTERIZE THE TOLERANCE OF MICROORGANISMS TO BIOBURDEN REDUCTION METHODS TO FORMULATE A SOUND BEST PRACTICES STUDY FOR PLANETARY PROTECTION IMPLEMENTATION CAMPAIGNS. OUR GOAL IS TO UNDERSTAND WHICH BIOBURDEN REDUCTION PROCESSES YIELD THE HIGHEST EFFICACY WITH THE LOWEST COST. THIS INFORMATION, IN COMBINATION WITH DETAILED STUDIES ON CURRENTLY USED BIOBURDEN REDUCTION METHODS, IS VITAL TO NASA AND INTERNATIONAL SPACE AGENCIES FOR REDUCING/PREVENTING FORWARD CONTAMINATION ON MARS DURING THE MARS 2020 MISSION. MOREOVER, REFINED CLEANING PROTOCOLS COULD DRASTICALLY REDUCE ATLO COSTS AND PROJECT RESOURCES. Other Administrative Action $0 8/6/18 Not listed THE PRIMARY FOCUS OF THE PLANETARY PROTECTION GROUPS AT NASA AND JET PROPULSION LABORATORY (JPL) IS THE BIOLOGICAL SAMPLING OF SPACECRAFT TO ENSURE INTERNATIONAL PLANETARY PROTECTION REQUIREMENTS ARE MET. SPACECRAFT UNDERGO RIGOROUS BIOBURDEN (MICROBIAL LOAD) REDUCTION MEASURES PRIOR TO LAUNCH TO MEET THESE LIMITS. DESPITE ATTEMPTS BY JPL AND NASA TO REDUCE THE BIOBURDEN USING VARIOUS CLEANING REAGENTS AND METHODS MICROORGANISMS PERSIST ON THE SPACECRAFT. MOREOVER ALTHOUGH STUDIES ARE CONSTANTLY EXPANDING OUR UNDERSTANDING OF LIFE IN EXTREME ENVIRONMENTS IT IS STILL UNCLEAR WHETHER MICROORGANISMS FROM EARTH TRAVELING ON SPACECRAFT CAN SURVIVE THE EXTREME ENVIRONMENTS THAT EXIST ON OTHER PLANETARY BODIES. THIS INFORMATION IS OF PARAMOUNT IMPORTANCE TO THE CURRENT MARS 2020 MISSION TO SEEK LIFE OUTSIDE OF EARTH. OVER THE DECADES NASA HAS IMPLEMENTED A NUMBER OF DIFFERENT CLEANING METHODS BUT VALIDATION STUDIES ARE OFTEN LACKING AND CURRENT NASA BIOBURDEN REDUCTION PROCEDURES DO NOT YIELD THE BIOBURDEN LEVELS MANDATED FOR SPACECRAFT TO EXPLORE AREAS OF MARS DEEMED AS "SPECIAL REGIONS" WHERE LIFE IS MOST LIKELY TO EXIST. THE RESEARCH PROPOSED FOR THIS PROJECT AIMS TO 1.) ASSESS SURVIVABILITY OF MICROORGANISMS COLLECTED FROM MARS 2020 TO SIMULATED MARS CONDITIONS TO DETERMINE WHICH MICROORGANISMS ARE THE HARDIEST TO EXTREME PLANETARY CONDITIONS ON MARS AND OF MOST CONCERN TO PLANETARY PROTECTION; AND 2.) CHARACTERIZE THE TOLERANCE OF MICROORGANISMS TO BIOBURDEN REDUCTION METHODS TO FORMULATE A SOUND BEST PRACTICES STUDY FOR PLANETARY PROTECTION IMPLEMENTATION CAMPAIGNS. OUR GOAL IS TO UNDERSTAND WHICH BIOBURDEN REDUCTION PROCESSES YIELD THE HIGHEST EFFICACY WITH THE LOWEST COST. THIS INFORMATION IN COMBINATION WITH DETAILED STUDIES ON CURRENTLY USED BIOBURDEN REDUCTION METHODS IS VITAL TO NASA AND INTERNATIONAL SPACE AGENCIES FOR REDUCING/PREVENTING FORWARD CONTAMINATION ON MARS DURING THE MARS 2020 MISSION. MOREOVER REFINED CLEANING PROTOCOLS COULD DRASTICALLY REDUCE ATLO COSTS AND PROJECT RESOURCES. $0 8/6/18 Not listed THE PRIMARY FOCUS OF THE PLANETARY PROTECTION GROUPS AT NASA AND JET PROPULSION LABORATORY (JPL) IS THE BIOLOGICAL SAMPLING OF SPACECRAFT TO ENSURE INTERNATIONAL PLANETARY PROTECTION REQUIREMENTS ARE MET. SPACECRAFT UNDERGO RIGOROUS BIOBURDEN (MICROBIAL LOAD) REDUCTION MEASURES PRIOR TO LAUNCH TO MEET THESE LIMITS. DESPITE ATTEMPTS BY JPL AND NASA TO REDUCE THE BIOBURDEN USING VARIOUS CLEANING REAGENTS AND METHODS, MICROORGANISMS PERSIST ON THE SPACECRAFT. MOREOVER, ALTHOUGH STUDIES ARE CONSTANTLY EXPANDING OUR UNDERSTANDING OF LIFE IN EXTREME ENVIRONMENTS, IT IS STILL UNCLEAR WHETHER MICROORGANISMS FROM EARTH, TRAVELING ON SPACECRAFT, CAN SURVIVE THE EXTREME ENVIRONMENTS THAT EXIST ON OTHER PLANETARY BODIES. THIS INFORMATION IS OF PARAMOUNT IMPORTANCE TO THE CURRENT MARS 2020 MISSION TO SEEK LIFE OUTSIDE OF EARTH. OVER THE DECADES NASA HAS IMPLEMENTED A NUMBER OF DIFFERENT CLEANING METHODS BUT VALIDATION STUDIES ARE OFTEN LACKING AND CURRENT NASA BIOBURDEN REDUCTION PROCEDURES DO NOT YIELD THE BIOBURDEN LEVELS MANDATED FOR SPACECRAFT TO EXPLORE AREAS OF MARS DEEMED AS "SPECIAL REGIONS" WHERE LIFE IS MOST LIKELY TO EXIST. THE RESEARCH PROPOSED FOR THIS PROJECT AIMS TO 1.) ASSESS SURVIVABILITY OF MICROORGANISMS COLLECTED FROM MARS 2020 TO SIMULATED MARS CONDITIONS TO DETERMINE WHICH MICROORGANISMS ARE THE HARDIEST TO EXTREME PLANETARY CONDITIONS ON MARS AND OF MOST CONCERN TO PLANETARY PROTECTION; AND 2.) CHARACTERIZE THE TOLERANCE OF MICROORGANISMS TO BIOBURDEN REDUCTION METHODS TO FORMULATE A SOUND BEST PRACTICES STUDY FOR PLANETARY PROTECTION IMPLEMENTATION CAMPAIGNS. OUR GOAL IS TO UNDERSTAND WHICH BIOBURDEN REDUCTION PROCESSES YIELD THE HIGHEST EFFICACY WITH THE LOWEST COST. THIS INFORMATION, IN COMBINATION WITH DETAILED STUDIES ON CURRENTLY USED BIOBURDEN REDUCTION METHODS, IS VITAL TO NASA AND INTERNATIONAL SPACE AGENCIES FOR REDUCING/PREVENTING FORWARD CONTAMINATION ON MARS DURING THE MARS 2020 MISSION. MOREOVER, REFINED CLEANING PROTOCOLS COULD DRASTICALLY REDUCE ATLO COSTS AND PROJECT RESOURCES. Not listed $150.0k 9/23/17 Not listed THE PRIMARY FOCUS OF THE PLANETARY PROTECTION GROUPS AT NASA AND JET PROPULSION LABORATORY (JPL) IS THE BIOLOGICAL SAMPLING OF SPACECRAFT TO ENSURE INTERNATIONAL PLANETARY PROTECTION REQUIREMENTS ARE MET. SPACECRAFT UNDERGO RIGOROUS BIOBURDEN (MICROBIAL LOAD) REDUCTION MEASURES PRIOR TO LAUNCH TO MEET THESE LIMITS. DESPITE ATTEMPTS BY JPL AND NASA TO REDUCE THE BIOBURDEN USING VARIOUS CLEANING REAGENTS AND METHODS MICROORGANISMS PERSIST ON THE SPACECRAFT. MOREOVER ALTHOUGH STUDIES ARE CONSTANTLY EXPANDING OUR UNDERSTANDING OF LIFE IN EXTREME ENVIRONMENTS IT IS STILL UNCLEAR WHETHER MICROORGANISMS FROM EARTH TRAVELING ON SPACECRAFT CAN SURVIVE THE EXTREME ENVIRONMENTS THAT EXIST ON OTHER PLANETARY BODIES. THIS INFORMATION IS OF PARAMOUNT IMPORTANCE TO THE CURRENT MARS 2020 MISSION TO SEEK LIFE OUTSIDE OF EARTH. OVER THE DECADES NASA HAS IMPLEMENTED A NUMBER OF DIFFERENT CLEANING METHODS BUT VALIDATION STUDIES ARE OFTEN LACKING AND CURRENT NASA BIOBURDEN REDUCTION PROCEDURES DO NOT YIELD THE BIOBURDEN LEVELS MANDATED FOR SPACECRAFT TO EXPLORE AREAS OF MARS DEEMED AS "SPECIAL REGIONS" WHERE LIFE IS MOST LIKELY TO EXIST. THE RESEARCH PROPOSED FOR THIS PROJECT AIMS TO 1.) ASSESS SURVIVABILITY OF MICROORGANISMS COLLECTED FROM MARS 2020 TO SIMULATED MARS CONDITIONS TO DETERMINE WHICH MICROORGANISMS ARE THE HARDIEST TO EXTREME PLANETARY CONDITIONS ON MARS AND OF MOST CONCERN TO PLANETARY PROTECTION; AND 2.) CHARACTERIZE THE TOLERANCE OF MICROORGANISMS TO BIOBURDEN REDUCTION METHODS TO FORMULATE A SOUND BEST PRACTICES STUDY FOR PLANETARY PROTECTION IMPLEMENTATION CAMPAIGNS. OUR GOAL IS TO UNDERSTAND WHICH BIOBURDEN REDUCTION PROCESSES YIELD THE HIGHEST EFFICACY WITH THE LOWEST COST. THIS INFORMATION IN COMBINATION WITH DETAILED STUDIES ON CURRENTLY USED BIOBURDEN REDUCTION METHODS IS VITAL TO NASA AND INTERNATIONAL SPACE AGENCIES FOR REDUCING/PREVENTING FORWARD CONTAMINATION ON MARS DURING THE MARS 2020 MISSION. MOREOVER REFINED CLEANING PROTOCOLS COULD DRASTICALLY REDUCE ATLO COSTS AND PROJECT RESOURCES. $150.0k 9/23/17