Whole Genome BiSUlfate Sequencing, goes with Existing research

Added: Mar 29, 2017 11:19 am The U.S. Department of Agriculture ( USDA ), Agriculture Research Service ( ARS ), intends to award a sole source, fixed price purchase order to the small business vendor: Novogene Corp., Inc. , located at 823 Anchorage Place, Suite 105, in Chula Vista, California, 91914-4534 whose Dun and Bradstreet Number is 07989805. The applicable North American Industrial Classification Code (NAICS Code) is: 541711 with a small business size standard of 1,000 employees. The proposed award is being issued under Authority of FAR 6.302-1 ( b ) ( i ), only one responsible source and no other supplies or services will satisfy the agency requirement.

USDA's Requirement is as follows:

The above stated vendor shall construct whole genome bisulfate sequencing "WGBS" Libraries using somatic tissue-genomic DNA from Holstein bulls using a customized protocol. The above stated vendor shall sequence the libraries through multiplexing multiple libraries per lane using a Illumina, Inc. Manufactured Hi-Seq platform and obtain an average of 50 Gb data per sample "Q30 Greater than or equal to 80 percent" using paired-end reads of 150 bp.. The above stated vendor shall perform basic bioinformatics analysis, including adapter pollution removal, low quality reads filtering, and production statistics. The specific reason why the above requirement will be awarded as a sole-source procurement, is because USDA was previously provided "existing" WGBS data on 30 cattle tissues by the above stated vendor, and a "identical" product is required to maintain research continuity.

THIS NOTICE INTENT IS NOT A REQUEST FOR QUOTATIONS. A determination by the Government not to compete this requirement based on responses to this notice is solely within the discretion of the Government. The purchase made will be made under simplified acquisition procedures, other than Full and Open Competition authorized by FAR 6.302-1, and Statutory Authority: 10 USC 2304 (C ) ( 1) as implemented by FAR 6.302-1. Oral Communication are not acceptable in response to this notice. This notice is for informational purposes only and is not to be construed as a commitment by the Government. The anticipated award dated is April 10, 2017.