Single Nucleotide Polymorphism Genotyping

Added: Jun 22, 2016 12:50 pm    

This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; quotes are being requested and a written solicitation will not be issued. Solicitation number AG-32SC­ S-16-0039 is issued as a request for proposal (RFP). The solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisit ion Circular 2005-88.   This solicitation will be a 100% Total Small Business Set-Aside. The associated NAICS code is 541711 (Research and Development in Biotechnology), with a small business size standard of 1000.

This acquisition is for the following item as identified in the Contract Line Item Number (CLIN):

 

 

0001) Single nucleotide polymorphism genotyping of 2,304 rainbow trout DNA samples

              

               Unit Price: ___________

              

               Quantity:  ___________

 

               Total Price:  ___________

 

0002) OPTION Single nucleotide polymorphism genotyping of 1,152 rainbow trout DNA samples

              

               Unit Price: ___________

              

               Quantity:  ___________

 

               Total Price:  ___________

 

              

Technical Specifications:

 

STATEMENT OF WORK - The USDA NCCCWA requires single nucleotide polymorphism genotyping of 2,304 rainbow trout DNA samples with an option for an additional 1,152 using Axiom myDesign TG arrays for single nucleotide polymorphism (SNP) genotyping in rainbow trout in the 384-well format (O_my_50K ) on the GeneTitan Instrument and or equal. Barcodes or barcoded plates are required for the Government provided DNA samples. Deliverable will include the genotyping data for all the samples and the raw data files in .eel format (CEL files) .All data must be provided on external hard drives. Contractor shall provide all services, supplies, equipment and facilities necessary to provide the deliverable specified in accordance with the terms, conditions, and specifications contained in the solicitation.

 

 

The following performance elements and benchmarks are required:

 

 

•1.   Amount of DNA per sample required is 200 ng of total rainbow trout genome DNA;

•2.    Minimum sample pass rate of 95%;

•3.    Minimum average SNP call rate of passing samples must be a minimum of 99%;

•4.    Minimum of 95% of SNPs must have SNP call rates greater than or equal to 97% over the passing samples;

•5.    Vendor must have a fully integrated LIMS (laboratory Information Management System) for sample tracking to ensure that there are no errors in matching the genotypes to the samples. Vendor must provide barcodes or barcoded plates for the DNA samples;

•6. Vendor must have in place a series of quality control (QC) steps during the SNP genotype processing.

•7. Complet ion and return of required data is required within 30 days of DNA samples receipt.

 

 

DELIVERABLE SHIPMENT AND DOCUMENTATION - Shipment shall be FOB Destination to USDA-NCCCWA

11861 Leetown Road

Kearneysville, WV 25430.

 

 

Deliverable will include the genotyping data for all the samples and the raw data files in .eel format (CEL files). All data must be provided in external hard drives to the USDA-NCCCWA , 11861 Leetown Road, Kearneysville,VVV 25430 . The order number shall be included on all documentation and shipment.

The Government anticipates award of a Firm Fixed Price contract.

 

 

 

Quoters responding to this announcement shall submit their quote in accordance with FAR 52.212-1. Submission of quote shall include the following: (1) Technical Specifications and (2) Price (to include shipping) .

 

 

The basis for award is Lowest Price Technically Acceptable (LPTA)."LPTA" means the expected outcome of the acquisition that, results from selection of the technically acceptable proposal with the lowest evaluated price.

Pursuant to FAR 52.212-2 the criteria for evaluation are: (1) Technical Specifications , and (2) Price (to include shipping).

 

 

Options. The Government will evaluate offers for award purposes by adding the total price for all options to the total price for the basic requirement. The Government may determine that an offer is unacceptable if the option prices are significantly unbalanced . Evaluation of options shall not obligate the Government to exercise the option (s).

 

 

INSPECTON AND ACCEPTANCE TERMS: Supplies will be inspected by Contracting Officer Representative (COR) and accepted at destination.

 

 

The following Federal Acquisition Regulation (FAR) and the Department of Agriculture Acquisition Regulation (AGAR) provisions and clauses apply to this acquisition:

FAR 52.204-7 Central Contractor Registration; FAR 52.204-10 Reporting Executive Compensation and First-Tier Subcontract Awards; AGAR 452 .204-70 Inquiries; FAR 52.209-6 Protecting the Government's Interests when Subcontracting with Contractors Debarred, Suspended or Proposed for Debarment;

FAR 52.212-1 Instructions to Offers-Commercial Items; FAR 52.212-2 Evaluation-Commercial Items (filled in as follows : Technical specifications and price); FAR 52 .212-4 Contract Terms and Conditions-Commerci al Items; FAR 52.212-5 Contract Terms and Conditions Required to Implement Statutes of Executive Orders-Commerci al Items; FAR 52.219-6 Notice of Total Small Business Set-Aside; FAR 52.219-28 Post Award Small Business Program Representation; AGAR 452 .219-70 Size Standard and NAICS Code Information; FAR 52.222-3 Convict Labor; FAR 52.222-19 Child Labor - Cooperation with Authorities and Remedies; FAR 52.222-21 Prohibition of Segregated Facilities; FAR 52.222-26 , Equal Opportunity Employment; FAR 52.222-36 Affirmative Action for Workers with Disabilities; FAR 52.222-50 Combating Trafficking  in Persons; FAR 52.223-18 Encouraging Contractor Policy to Ban Text Messaging While Driving; FAR 52.225-1 Buy American--Supplies; FAR 52.225-13 Restrictions on Certain Foreign Purchases; FAR 52.225-25 Prohibitions on Contracting with Entities Engaging in Certain Activities or Transactions Relating to Iran-Reps and Certs; FAR 52.232-33 Payment by Electronic Funds Transfer-Central Contractor Registration; FAR 52.233-3 Protest After Award ; FAR 52.233-4 Applicable Law for Breach of Contract; FAR 52.252-1 Solicitations Provisions Incorporated by Reference; FAR 52.252-2 Clauses Incorporated by Reference.