Single fluorescent molecule detection, multi-parameter imaging microscope for single nanoparticle (>20 nm) detection.

**** 8/20/2019 SF-30 File Attached with Amendment and answer to questions *****
This is a combined synopsis/solicitation for commercial items, prepared in accordance with format in FAR 12.6 as supplemented with additional information included in this notice. This announcement constitutes the only solicitation and a separate written solicitation will not be issued. This solicitation: No. RFQ-NCI-5372271 includes all applicable provisions and clauses in effect through FAR Federal Acquisition Circular (FAC) 2019-02 (June 2019) simplified procedures for commercial items.
This requirement is assigned North American Industry Classification System (NAICS) code 333314 with a size standard of 500 Employees is being considered.
Only one award will be made because of this solicitation. This will be awarded as a firm fixed price type contract.

BACKGROUND

The U.S. Department of Health and Human Services, National Institutes of Health, National Cancer Institute (NCI), Center for Cancer Research (CCR). The intent of this purchase is to acquire a microscope, for the purpose of obtaining very high-resolution data with extremely high sensitivity for the analysis of submicron extracellular vesicles (EVs). The purpose of the instrument is to be able to perform studies that will identify and characterize single EVs and single fluorescent molecules across multiple lasers. This ultra-high level of sensitivity and precision is required for tests that will detect and enumerate EVs that have as few as a single molecule of interest. The purpose of this instrument is to provide single-molecule resolution for EV analyses as part of the EV analysis pipeline and the characterization of nanomaterials that is integral to the research that is being conducted in the Translational Nanobiology Section of the Laboratory of Pathology.

The salient characteristics that apply to this acquisition are as follows:
• Single nanoparticle (>20 nm) detection
• Single fluorescent molecule detection across multiple fluorophores
• Single particle number concentration enumeration
• Permanent alignment
• Ability to perform all operations on a standard desktop without the need for a vibration- isolating optical table
• Autofocus system
• Class 1 laser safe device
• Microscope unit and light engine with less than 465 cm2 and 810 cm2 respectively in footprint * Real-time calibrated power readings at the sample plane
• Optional integrated microfluidics upgrade including built in software for programmed delivery
• Two modes of operation to achieve super-resolution: single-molecule localisation microscopy (SMLM) and multifocal structured illumination microscopy (mSIM)
• Resolution by SMLM xy: 20 nm; z: 50 nm
• Three modes of illumination: widefield, TIRF and HILO
• Simultaneous dual emission channels
• 50×80 μm homogeneous field of view
• Homogeneous top-hat illumination
• Advanced piezo stage driven in X, Y and Z with 1 nm steps and 30 nm reproducibility
• Real-time (GPU) localization and quantitative analysis
• Whole-body temperature control at 37°C
• sCMOS Hamamatsu orca flash 4 V3, with high quantum efficiency 82% and high full frame acquisition rate 100fps
• Unlimited software licenses. This includes unlimited lifetime upgrades and free licenses for data analysis
• A minimum of 12-month manufacturer warranty. Online support for the first 12 months after commissioning, if needed.
• Must include training and installation
• Quote must include shipping, including (FBO)and installation cost. NCI will not be responsible for any cost not included.
Note: NCI plans to award a contract that has FOB origin delivery terms.
PAYMENT
Payment shall be made upon the delivery, installation, acceptance and completion of training.

DELIVERY / INSTALLATION
The Contractor shall deliver and install the required item(s) within 30 days of purchase order award to the following location:

10 Center Drive
10CRC
Bethesda, MD 20892

The Contractor that receives the award will be provided with the complete mailing address and point of contact. Upon award, contractor must notify the NCI Technical Point of Contact to schedule the delivery.

CONTRACT CLAUSES/PROVISIONS
The following Federal Acquisition Regulation and Health & Human Services Acquisition
Regulation Provisions/Clauses apply to this acquisition:

FAR 52.252-2 CLAUSES INCORPORATED BY REFERENCE (FEB 1998)

This contract incorporates one or more clauses by reference, with the same force and effect as if they were given in full text. Upon request, the Contracting Officer will make their full text available. Also, the full text of a clause may be accessed electronically at this/these address(es): http://www.acquisition.gov/far/ and/or http://www.hhs.gov/policies/hhsar/.

Clause No. Title

FAR 52.212-1 INSTRUCTIONS TO OFFERORS-COMMERCIAL ITEMS (OCT
2018
FAR 52.212-2 EVALUATION COMMERCIAL ITEMS (OCT 2014)
FAR 52.212-3 OFFEROR REPRESENTATIONS AND CERTIFICATIONS-
COMMERCIAL ITEMS (OCT 2018)
FAR 52.212-4 CONTRACT TERMS AND CONDITIONS-COMMERCIAL
ITEMS (OCT 2018)
HHSAR 352.222-70 CONTRACTOR COOPERATION IN EQUAL
EMPLOYMENT OPPORTUNITY (DEC 2015)
FAR 52.212-5 CONTRACT TERMS AND CONDITIONS REQUIRED TO
IMPLEMENT STATUTES OR EXECUTIVE ORDERS-
COMMERCIAL ITEMS (JAN 2019).

The following FAR clauses cited in paragraph (b) of the clause at FAR 52.212-5 are also applicable to this acquisition:

Clause No. Title

FAR 52.203-6 Restrictions on Subcontractor Sales to the Government (Oct 1995)
FAR 52.203-13 Contractor Code of Business Ethics and Conduct (Oct 2015)
FAR 52.204-10 Reporting Executive Compensation and First Tier Subcontract Awards (Oct 2018)
FAR 52.209-6 Protecting the Government's Interest When Subcontracting with
Contractors Debarred, Suspended, or Proposed for Debarment
(Oct 2015)
FAR 52.209-9 Updates of Publicly Available Information Regarding
Responsibility (Oct 2018)
FAR 52.219-8 Utilization of Small Business Concerns (OCT 2018)
FAR 52.219-28 Post Award Small Business Program Representation (JUL2013)
FAR 52.222-3 Convict Labor (APR 2015)
FAR 52.222-19 Child Labor-Cooperation with Authorities and Remedies (JAN 2018)
FAR 52.222-21 Prohibition of Segregated Facilities (SEP 2016)
FAR 52.222-26 Equal Opportunity (SEP 2016)
FAR 52.222-35 Equal Opportunity for Special Disabled Veterans, Veterans of
the Vietnam Era, and Other Eligible Veterans (JAN 2019)
FAR 52.222-36 Equal Opportunity for Workers with Disabilities (JUL 2014)
FAR 52.222-37 Employment Reports of Special Disabled Veterans, Veterans of
the Vietnam Era, and Other Eligible Veterans (FEB 2016)
FAR 52.222-40 Notification of Employee Rights Concerning Payment of Union
Dues or Fees (DEC 2010)
FAR 52.223-18 Encouraging Contractor Policies to Ban Text Messaging while
Driving (AUG 2011)
FAR 52.225-3 Buy American Act -Free Trade Agreements - Israeli Trade
Act (JUN 2009)
FAR 52.225-5 Trade Agreements (AUG 2009)
FAR 52.225-13 Restrictions on Certain Foreign Purchases (JUN 2008)
Far 52.232-33 Payment by Electronic Funds Transfer-System for Award
Management (OCT 2018)
FAR 52.211-6 BRAND NAME OR EQUAL (AUG 1999)

(a) If an item in this solicitation is identified as "brand name or equal," the purchase description reflects the characteristics and level of quality that will satisfy the Government's needs. The salient physical, functional, or performance characteristics that "equal" products must meet are specified in the solicitation.
(b) To be considered for award, offers of "equal" products, including "equal" products of the brand name manufacturer, must-

(1) Meet the salient physical, functional, or performance characteristic specified in this solicitation;
(2) Clearly identify the item by-
(i) Brand name, if any; and
(ii) Make or model number;
(3) Include descriptive literature such as illustrations, drawings, or a clear reference to previously furnished descriptive data or information available to the Contracting Officer; and
(4) Clearly describe any modification the offeror plans to make in a product to make it conform to the solicitation requirements. Mark any descriptive material to clearly show the modification.

(c) The Contracting Officer will evaluate "equal" products based on information furnished by the offeror or identified in the offer and reasonably available to the Contracting Officer. The Contracting Officer is not responsible for locating or obtaining any information not identified in the offer.

(d) Unless the offeror clearly indicates in its offer that the product being offered is an "equal" product, the offeror shall provide the brand name product referenced in the solicitation.

HHSAR 352.203-70 ANTI-LOBBYING (DEC 2015)

Pursuant to the current HHS annual appropriations act, Public Law 112-74, except for normal and recognized executive-legislative relationships, the Contractor shall not use any HHS contract funds for:

(a)Publicity or propaganda purposes;
(b)The preparation, distribution, or use of any kit, pamphlet, booklet, publication, electronic communication, radio, television or video presentation designed to support or defeat the enactment of legislation before the Congress or any State or local legislature or legislative body, except in presentation to the Congress or any State or local legislature itself; or designed to support of defeat any proposed or pending regulation, administrative action, or order issued by the executive branch of any State or local government, except in presentation to the executive branch of any State or local government itself; or
(c)Payment of salary or expenses of the Contractor, or any agent acting for the Contractor, related to any activity designed to influence the enactment of legislation, appropriations regulation, administrative action, or Executive order proposed or pending before the Congress or any State government, State legislature or local legislature or legislative body, other than for normal and recognized executive-legislative relationships or participation by an agency or officer of a State, local or tribal government is policymaking and administrative processes within the executive branch of that government.

The prohibitions in subsections (a), (b), and (c) above shall include any activity to advocate or promote any proposed, pending, or future Federal, State or local tax increase, or any proposed, pending, or future requirement for, or restriction on, any legal consumer product, including its sale or marketing, including, but not limited to, the advocacy or promotion of gun control.

SUBMISSION INFORMATION

Proposals must be submitted on an SF-1449 with a completed 52.212-3 Offeror Representations and Certifications-Commercial Items-with DUNS Number Addendum, signed by an authorized representative of the offeror OR provide a copy of valid certification in the System for Award Management (SAM) at www.sam.gov.

Questions must be submitted electronically (via email) to Contracting Officer, Lisa Hill at lisa.hill@nih.gov MS Word or Adobe Portable Document Format (PDF), no later than August 15th, 11:00 am, EST.

Quotes must be submitted electronically (via email) to in MS Word or Adobe Portable Document Format (PDF), no later than August 23, 2019; 11:00 am, EST. All responses must be received by the specified due date and time to be considered. QUOTES RECEIVED AFTER THIS DATE AND TIME WILL NOT BE CONSIDERED. No collect calls will be accepted.

Please refer to solicitation no: RFQ-NCI-5372271 in all correspondence.
EVALUATION

The award decision will be based on a Lowest Price Technically Acceptable (LPTA) determination. Award will be made on all or none basis. If you are providing an "equal" product, you must submit additional documentation that highlights and describes how your offering is equal in accordance with FAR Clause 52.211-6.

PRICE PROPOSAL

The Offeror shall prepare a price proposal that contains all information necessary to allow for a comprehensive evaluation of the prices quoted. Specifically, your quote must include the following: Federal Tax Identification Number (TIN); Data Universal Numbering System (DUNS) Number; and Business Size (e.g., small or large).

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