Pacbio Sequencing of 8 citrus (Pomelo) tissue samples

This award is for a Genomics Project of Pacbio Sequencing of 8 citrus (Pomelo) tissue samples that must run together as a batch of 8.

Receive 8 tissue samples and extract HMW DNA suitable for Pacbio sequencing

a-

Must receive correct amount of tissue and quality of tissue for HMW DNA;

b-

Extractions performed using modified CTAB; QC data available

c-

cost for shipping is the responsibility of the customer

Produce Pacbio HiFi sequence libraries

a-

Construct one HiFi library from each DNA sample

b-

HiFi library, size selected 15-25kb

c-

Amnt of library correct for Number of cells to run

Sequence libraries on Pacbio Sequel II; FOR EACH SAMPLE:

a-

Sequencing Chemistry used is SeqII v2.0; SMRT cells are 8M v1

b-

Sequence 1 cell HiFi CCS (30hr)

c-

Each CCS cell is expected to produce 16-24Gb of QV20+ CCS data. No guarantee on yield.

Run CCS analysis on all raw data; summarize data and deliver via secure FTP

a-

CCS analysis run on each HiFi CCS cell raw data;

b-

No additional bioinformatics. No Assembly

c-

AGI QC data, monitor progress, summarize outputs and stats. Deliver CCS data via secure FTP (or cloud).

TOTAL COST for 8 sample data sets (from 8 cells sequenced)

Includes all materials, supplies, labor, and fees

Awarded contract 12405B21P0080 to UNIVERSITY OF ARIZONA for 37200.00 on 2021-03-29.