ME220 Focused-ULTRASONICATOR

This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR 12.6 as supplemented with additional information included in this notice. This announcement constitutes the only solicitation and a separate written solicitation will not be issued. This solicitation number is RFQ-75N94019Q00077 and is issued as a Request for Quotation (RFQ). The solicitation /contract will include all applicable provisions and clauses in effect through Federal Acquisition Circular 2019-03. The North American Industry Classification (NAICS) Code is 334516 with a size standard of 1000. This acquisition is being conducted using Simplified Acquisition Procedures in accordance with FAR Part 13. The National Institutes of Health (NIH), National Institute of Digestive, Diabetes & Kidney Diseases (NIDDK) has a requirement to procure a ME220 Focused-ultrasonicator.

Our ongoing research at the NIDDK studies biological functions of thyroid hormone receptor beta gene (Thrb) that requires to make next generation sequencing libraries using tiny amount of RNA from either single cell or small tissue including retina/cochlear sensory neurons, adrenal gland and pituitary. Our current research topics include the study of specific selection of these small-population cells or developmental transcriptome analyses from Thrbexpressing tissues/cells. Single-cell sequencing technique, which is powered by recently developed nextgeneration sequencing (NGS), enables the study of transcription factor binding site, transcriptomic analysis, epigenomic modification changes and chromatin structure. Our lab developed a single cell capturing technique from tiny TRb-population tissue to investigate transcriptomic analysis of individual Thrb positive cells. Among
series procedures involved in the RNA-seq library construction, the step of cDNA fragmentation is critical, in which evenly and efficiently sonication of the cDNA strands into desired size is the key factor to ensure high quality libraries and optimum data.
We would like to purchase the ME220 Focused-ultrasonicator to shear the tiny RNA or DNA sample from single cell or small tissue for investigating the roles of thyroid hormone receptor beta and epigenomic regulators. This ME220 provides real-time isothermal, non-contact batch processing of 1 to 8 samples. This is important to maintain the integrity of the chromatin/nucleic acids or RNA for making next generating sequencing libraries. This equipment fits our requirements on reduction of processing times, sample-to-sample reproducibility, operating outside the human audible range, sample input flexibility and rapid high-throughput data acquisition.

Details:
1 530194 Promotional Credit
1 500506 ME220 Focused-ultrasonicator
1 520237 truChIP Chromatin Shearing Tissue Kit with Formaldehyde
1 520045 microTUBE AFA Fiber Pre-Slit Snap-Cap 6x16mm (25)
1 500522 ME220 Rack 4-place microTUBE Screw Cap
1 520225 8 AFA-TUBE TPX Strip (12)
1 500613 8 AFA-TUBE TPX Strip Caps
1 520108 8 microTUBE Strip Foil Seal (12)
1 500609 ME220 Rack 8 AFA-TUBE TPX Strip
1 500514 ME220 Rack - Snap-Cap/Crimp-Cap/8 microTUBE Strip V1
1 500520 ME220 Rack - 4 place milliTUBE
1 520135 milliTUBE 1 ml AFA Fiber (24)
1 500526 ME220 Waveguide 8 Place
1 500534 ME220 Waveguide 4 Place
2 500523 ME220 Rack Loading Station
2 520200 ME220 AFA-grade Water (1L)
1 500529 ME220 Particle Filter and Gasket Replacement Kit
1 530013 Installation & Training
1 SHIPPING Shipping

The offeror must include a completed copy of the following provisions: 1) FAR Clause 52.212-1 Instructions to Offerors - Commercial items; 2) FAR Clause 52.212-2, Evaluation - Commercial Items. As stated in FAR Clause 52.212-2 (a) The Government will award a contract resulting from this solicitation to the responsible offeror whose offer conforming to the solicitation will be advantageous to the Government, price and other factors considered. The following factors will be used equally to evaluate offers: Technical Evaluation, Price, and Past Performance. The Government will make award based on Best Value.

Note: Past Performance Information: Vendors must submit a listing of the most recent contracts/awards (minimum of 3) which demonstrate similar work in nature to this Solicitation. Contracts/awards may include those entered with the Federal Government, state and local governments and commercial concerns. Include the following information for each contract or subcontract:

1. Name of Contracting Organization
2. Contract Number (for subcontracts provide the prime contract number and the subcontract number)
3. Contract Type
4. Total Contract Value
5. Description of Requirement
6. Contracting Officer's Name and Telephone Number
7. Program Manager's Name and Telephone Number

3) FAR Clause 52.212-3, Offeror Representations and Certifications - Commercial Items; 4) FAR Clause 52.212-4, Contract Terms and Conditions - Commercial Items; 5) FAR Clause 52-212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders - Commercial Items - Deviation for Simplified Acquisitions. The Dun and Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN) and the certification of business size shall be included. The clauses are available in full text at http://www.arnet.gov/far.

Interested vendors capable of providing the Government with the items specified in this synopsis should submit their quotation to the below email address. Quotations will be due 10:00AM EST August 28, 2019. Offerors shall send the quotation/proposal to Verne Griffin at verne.griffin@nih.gov. The quotation must reference Solicitation number RFQ-75N94019Q00077. All responsible sources may submit a quotation, which if timely received, shall be considered by the agency. Responses to this notice shall contain sufficient information to establish the interested parties' bona-fide capabilities for fulfilling the requirement and include: unit price, list price, shipping and handling costs, the delivery period after contract award, the prompt payment discount terms, the F.O.B. Point (Destination or Origin), the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov."

Faxed copies will not be accepted.

.