Kinetic Exclusion Assay instrument

Added: Sep 05, 2017 7:57 pm Provide KinExA 3100 or equivalent, such that the instrument will:

Measure interactions in which both molecules are unmodified by labels, tags, immobilization, etc.

Measure binding interaction where both molecules are free in solution, not to immobilized surfaces, which is a more physiologically relevant measurement.

Is capable of measuring a wide range of binding constants as listed below:
   Kd Dynamic Range(M): 1e-15 - 1e-7
   Association Rate Range((M-1s-1): 1e4 -1e9
   Dissociation Rate Range (s-1): 1e-1 -1e-7

Has the ability to measure affinity and kinetics of molecules to intact engineered and native cells.

Is capable of measuring molecules in a wide array of matrices including serum, crude cell lysates, raw environmental samples, media and buffering solutions which include solvents e.g. DMSO, pyridine, etc. 

Has single click analysis which includes true 95% confidence intervals with the ability to measure complex binding.

Abiity to measure and monitor internal flow pressure throughout an experiment.

Measurements are not limited by the size of the interacting partner-will work with ions to whole cells.

Also provide Red Filter Set, Appropriate software, Standard instrument accessory kit, one year warranty, training for one person and installation.