CellInsight CX7 High Content Analysis Platform

Added: May 30, 2018 6:51 am Department of Health and Human Services, Eunice Kennedy Shriver, National Institute of Child Health and Human Development, 6710B Rockledge Drive, Room 1155A, Bethesda, MD 20817. National Institute of Diabetes and Digestive Kidney Diseases (NIDDK) plans to procure on a sole source basis with Life Technologies Corporation, 3175 Staley Road, Grand Island, NY 14072 USA for CellInsight CX7 High Content Analysis Platform.

NIDDK has been developing Beta-cell Stem Cells from the American Native population. During differentiation, cells have certain biomarkers that are detected during Development. This is to assure the researchers that they have the cells at the proper stage and not a mixture. When the cells become confluent, there is a chance that there may be a mixture of different cells. The flow cytometer has been used to detect that the stem cells are at the proper stage of development. As the cells grow, the number of cells become very large. It has been shown that it is necessary to identify these cells and their processes in real time. The images obtain need to be used for segmentation and quantification.
The proposal intends to: 1) make the chamber results more accurate, 2) make the chamber more stable over time, 3) reduce chamber downtime, and 4) enhance the ability to fix the chamber when it is malfunctioning.

Task Area 1
White Glove delivery (instrument is delivered to the Lab, removal of shipping debris, installation, and training. Vendor must retain a Broker to complete any customs documents, or other import paperwork necessary to ensure that the shipment clears US Customs.

Task Area 2
Testing of instrument to assure proper operation. Shipping company must contact Project Manager at 602 XXX XXXX to schedule the delivery to NIH, NIDDK Phoenix, AZ 85004. (Building and Room # will be provided at time of award)

Task Area 3
Warranty 1 year - Project Manager must inspect the delivery PRIOR to signing for delivery, to ensure that there is no damage to packaging and/or product. If Project Manager is unable to inspect product at the time of delivery, Project Manager will sign for delivery with a "PENDING INSPECTION" notation on the bill of lading/packing slip.

Task Area 4
Software updates from vendor.

Features of the instrument
Optical train-the custom inverted epifluorescence optical train incorporates a dual patterned spinning-disk
Light source-the CellInsight CX7 HCA Platform is equipped with a solid-state,
Transmitted light imaging-a 4-channel LED array for bright-field illumination
Confocal imaging-high-speed CrESTTM spinning disk confocal technology
Emission filters-five-color dichroic and emission filter pair allows multiplexing of up to 5 channels simultaneously
Camera-the PhotometricsTM X1 CCD camera

Objectives-standard Olympus objectives
Plate and slide compatibility-the CellInsight CX7 HCA
Custom configuration-a software tool allows setup of custom plate and slide formats that comply with SBS standards
Focus methods-the CellInsight CX7 HCA Platform uses proprietary software-based autofocus and laser-based autofocus
Robotic integration-the Thermo ScientificTM OrbitorTM RS Microplate Mover controlled by the Thermo ScientificTM HCS StudioTM

Expected deliverables:

CellInsightTM CX7 High Content Analysis Platform includes:
1. CELLINSIGHT CX7 HCA READER
2. ONSITE-1 DAY TRAINING
3. ONSITE NEW USER TRAINING
4. HCS Studio Software for client supplied computer. Includes single seat perpetual license of HCS Studio to all applications supported by the instrument configuration. Requires supported minimum computer specifications. Separate installation fee may apply.
5. ORBITOR RS ROBOT -CX7ONLY
6. HCA ONSTAGE INCUBATOR CX7. Onstage Incubator for CellInsight CX7 High Content Analysis Platforms with built in scheduling, well level and cell level kinetic analysis.
7. AB PROTECTION, CX7 READER
8. STORE STANDARD EDITION. HCA image data management software. Compatible with SQL Server 2008/Oracle. Includes license connecting up to 1 HCA platform and 1 application server software. Requires minimum server and database specifications supplied by customer. Customer installable.
9. Installation and Training provided to determine if instrument is fully functional.

In compliance with Federal Acquisition Regulation (FAR) 13.106-1(b) Soliciting from a single source. This notice of intent is not a request for competitive proposals, however; all responses received, before the closing date, of this announcement will be considered by the Government. A determination by the Government not to compete this proposed acquisition is based upon responses to this notice and is solely within the discretion of the Government. It is anticipated that the RFQ NICHD201800042 will be available ten (10) days after the publication date of this synopsis. Receipt of quotations will be due five (5) days after the release of the RFQ. All interested parties to this solicitation may request a copy of the RFQ package via email verne.griffin@nih.gov.

Responses to this notice shall contain sufficient information to establish the interested parties' bona-fide capabilities for fulfilling the requirement and include: unit price, list price, shipping and handling costs, the delivery period after contract award, the prompt payment discount terms, the F.O.B. Point (Destination or Origin), the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov." Third party repair vendor to provide a signed agreement with the OEM to provide certified technicians and OEM parts.

All requests must contain offerors name, address, telephone number and fax number.