Cell Sorter

This is a Source Sought Request for Information (RFI) for brand name of or equal to the FACSMelody fluorescence-activated cell sorting (FACS) machine for separation and isolation of Monocytes, T cells, and NK cells from blood samples (cell sorter) manufactured by Becton, Dickinson & Co.

Salient characteristics for the BD FACSMelody cell sorter (brand name or equal):
a) Benchtop cell sorter with completely automated setup, calibration and monitoring of laser and nozzle alignment, droplet breakoff, drop delay, side stream positioning, stream alignment and fluidics
b) Walkaway operation, including detection and prevention of bubble introduction into fluidics stream and collection container overflow
c) Multiple sorting modes from temperature-controlled, agitated, 12 x 75 mm sample tubes including 2-way, single and purity
d) 488 nm and 640 nm excitation lasers
e) Forward scatter, side scatter, and at least four fluorescence detectors including the following bandpass filters: 510/10 nm, 586/42 nm, 660/10 nm and 700/54 nm (downstream of a 665 nm long pass)
f) Ability to collect from single cell sorts into untreated 6-, 48- and 96-well plates with index sorting software that can display the flow cytometry data for each deposited cell
g) High sensitivity (ability to detect less than 50 MESF-PE and less than100 MESF-FITC) that allows detection and sorting of dim and rare cells
h) Windows 10 PC and monitor that meet minimum requirements for the software
i) Customized BSL2 biosafety cabinet
j) All consumables and replacement parts necessary for one year of operation (80 individual days of calibrations, cleanings and sorts of viable cells for downstream culturing) including sheath buffer and extra 100 micrometer nozzle
k) Air compressor if pressurized air supply is required and any air or water filters if required for a non-distilled water supply
l) Installation, training, and first-year service contract/warranty

Interested vendors with product(s) meeting these specifications shall submit product information to include spec data, pricing, delivery terms and conditions, CAGE and DUNS codes prior to the close of this RFI.

.