SPECIFICATIONS_WSIS.docx

Specifications Project Title: Whole-slide Imaging System DRD #: 2743 Date Submitted: 04 Nov 2014 Project POC: Angelo Madonna

Specifications required for this procurement include:

· Whole-slide Imaging System Microscope based scanning system providing enhanced flexibility in scanning techniques.

· System must interface with BX-51 Fluorescence Microscopes

· Utilizes multiple scanning objectives: 4x, 10x, 20x, 40x

· Has the ability to add 60x or 100x oil scanning

· Has the ability to scan in multiple Z‐planes

· Provides an Extended Focus Image scan (EFI) similar to a max projection

· The scanning system allows for the real time review of the high resolution scan during the scanning process

· The system can scan multiple ROI from one sample and save as a single file

· Pyramidal File Structure

· The system can accommodate sample slides of 1”x3”, 2”x3” or 3”x5” dimensions and larger with some adjustment

· The system is able to scan multiple magnification layers within the same image file

· The system is able to output image files to JP2000, .tiff or .bgt (bigtiff)

· The scanning system can be upgraded to include fluorescence scanning

· The scanning system supports both color and monochrome cameras

· The system must supports 16 bit monochrome image files

· Fluorescence calibration is available for each individual color channel

· The system provides real‐time online deblurring for fluorescence scanning applications

· The system is able to support advanced image processing software including maximum intensity projection and deconvolution algorithms

· Must come configured with a set of 8 position excitation and 8 position emission high speed filter wheels that allow rapid changeover between fluorochromes,

· The system utilizes a 14 bit Cooled monochrome CCD camera. Minimizing exposure times and delivering exceptional sensitivity and dynamic range.

· The VS-ASW FL software calculates a Z-focus offset during the calibration process to insure that each wavelength is optimally focused during multi-channel acquisition.

· Dark Field Microscopy capability

· Capable of Differential Interface Contrast and Polarized Light Microscopy