STELLARIS 5 Specs.pdf

Leica STELLARIS 5 Spectral Confocal System including:

• DMi8 CS Inverted Microscope ° Closed-Loop Focus 0 Binocular Tube, BF Detector ° Fluorescence Filter Cubes for DAPI, FIT C, and Rhodamine Visualization 0 High-Precision XV Scanning Stage

• Microscope Objectives for Confocal Scanning 0 5X dry, 10X dry, 20X dry, 40X oil, 63X oil

• STELLARIS 5 Scan Head with Leica's Low Angle Liachroic Beam Splitters

Tunable-Speed Conventional Scanner www.leica-microsystems.com 1 I 17

• Linear Scan Mode

• Scan speeds up to 2,600 Hz, 5,200 Hz Bidirectional

• Scan formats up to 8,192 x 8,192

• 10 fps x 5 simultaneous channels= 50 images per second @ 512 x 512

• 22mm FOV scanner with continuous zoom and scan field rotation

STELLARIS Solid State Lasers 405nm, 488nm, 561 nm, 638nm

Four (4) Simultaneous Spectral Detection Channels based on Leica's High-Efficiency Pellin

Broca Prism

• Power HyD S 0 New silicon-based, solid state detector with >56% Photon Detection Efficiency

(PDE)

0 Increased sensitivity in the NIR up to 850nm 0 >2-fold increase in dynamic range over previous generation HyD detectors 0 Analog and Photon Counting modes with PowerCounting®

• Premium CUDA Workstation with GPU for accelerated 3D image display and processing

• Leica Lightning for Near Real-T ime Confocal SuperResolution and Image Detail

Extraction (S/N improvement) 0 SuperResolution with resolution to 120nm lateral and 200nm axial 0 Lighting & Rolling Average with Resonant Scanning for maximum S/N and

SuperResolution at full speed

• Custom Height TMC Air Table for Vibration Isolation

• With Installation and Training

I Microscope Stand

# Items Quantity

DMi8 CS

155933662

Inverted research microscope based on the DMi8 series, with motorized nosepiece.

Prepared for confocal imaging.

-motorized fluorescence turret with 6 positions for filter cube and motorized shutter

-suitable for lenses with magnifications from 1.25x to 100x

-Microscope controller CTR Box Advanced

-SmartMove for easy control in xy and z

-Fluorescence Intensity Manager (FIM) www.leica-microsystems.com 2 I 11

Items Quantity

-All microscope parameters are displayed on the LCD

-Quick switching from coarse to fine drive

-Motorized 6x nosepiece

-Camera port (prepared) on the right side

-additional Infinity Port on the right side

-nosepiece with cooling system

-spill protection for motorized nosepieces on DMi8 with sealing ring and collecting container

-condenser S1/S28

-condenser head S28/0.55

-Optical equipment for with brightfield light source prepared for confocal microscopy

-eyepieces HC PLAN 10/25 BR M

-connection for lamp house, straight for DMI

-Immersion oil F

-Accessories and holder for confocal scanner

-Laser safety equipment

Transmission axis

155933666

Motorized transmission axis (without DLS) for optional photomultiplier allowing TLD

(software controlled)

Bino Tubus

155933660

Binocular tube for research microscope DMi8 CS inverted

Closed Loop Focus

158204201

Motorized focus drive for DMi8 with additional closed loop feedback for highest precision positioning.

BF detector for DMI

158004201

Transmitted light detector (TLD) for TCS SP8 DMI 6000 and DMI 6000 CEL Detector for acquisition of transmitted light images with interference contrast, phase contrast (DMI

6000 only) or bright field illumination (with laser scanning)

Constains

-Photomultiplier tube

-Motorized shutter

-Software controlled switching to TLD detection

Leica LED 3

158000677 www.leica-microsystems.com 3 I 11

# Items Quantity

LED3 is a mercury-free, bright, and versatile solid-state light source offering white light in the 390-680nm range and is well suited for a range of fluorescence microscopy and stereo microscopy applications. LED3 is practically maintainance-free and offers better output stability and longer life time of the lamp than traditional metal-halide lamps. LED3 can be triggered to synchronize illumination with camera exposure and minimize photobleaching.

The LED3 Bundle contains: 1x LED 3 light source, 1x 3mm Liquid-Light-Guide, 1x 24V DC

220W Power Supply, 1x 2mm Hex Allen Wrench, 1x BNC-to-SMB Cable, 1x Adapter plug

BNC-to-SMB

Scanning stage inv. universal

158004141

70 Motorised Scanningstage for DMl8 and DMI 6000 Travel range: 127 x 83mm Spindle pitch:

1 mm Speed: 10mm/sec Resolution: 0,02-0,04µm Reproducibility:< 1 µm Accuracy:+/- 3µm

The SuperZ Galvo stage has to be ordered separately. It is not included in the order number 158004141.

Cable Scanning Stage, 3 m, 90°

15500332

80 Extension cable for Scanning stages which are operated by CTR 6500. Length 3m, 90° angled Suitable for 156504150 Scanning stage upright 156504141 Scanning Stage inv.

Universal 156504150 Scanning stage upright 156504163 Scanning Stage CFS flat

10ML IMMERSION OILNON-FLUORESCING

15513859

XV advanced board for scanning stage

15525226

Univ.hold.frame K:0311.200

15600234

110 Universal Mounting frame invers Frame to fix different cultivation vessels (petri dishes with 24-68 mm diameter) and glass slides (with 24-120 mm length) with two moveable brackets with a variable clamping range. Outer dimension: 160x110 mm.

Filter cube LED 405

15525338

Filter Cube LED 405, size P

• Excitation: 405/60

• Dichroic: 455

• Emission: 470/40

Filter cube LED 470

15525340 www.leica-microsystems.com

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Filter Cube LED 470, size P

• Excitation: 470/40

• Dichroic: 495

• Emission: 525/50

Filter Cube RHOD LP

15525303

Items Quantity

Filter cube RHOD longpass Size: for DMi8 Excitation: BP 540/45 Emission: LP 590

I

HC PL FLUOTAR 5x/0.15

15506224

Items Quantity

Plan Fluotar objective with good color correction for standard fluorescence applications.

For use with and without coverglass, no immersion.

Qualified as illumination objective for the Digital LightSheet (DLS) Module in combination with the DLS TwinFlect 2.5mm (158007012).HC PL FLUOTAR 2.5x/0.07 (15506523) is required for DLS calibration.

Free working distance: 12.0 mm.

Obj. HC PL APO 10x/0.40 CS2

15506407

160 Objective HC PL APO 10x/0.40 CS2 Plan apochromatic objective with superior color correction CS2, optimized for confocal scanning applications. This objective is ideal for simultaneous use of 405 nm and visible excitation with the TCS SP8.

For use with 0.17 mm coverglass. Air immersion. Free working distance: 2.2 mm.

Obj. HC PL APO 20x/0.75 CS2

15506517

170 Plan apochromatic objective with superior color correction, optimized for confocal scanning applications.

For use with 0.17 mm coverglass, no immersion.

Free working distance: 0.62 mm.

Obj. HC PL APO 40x/1.30 Oil CS2

15506358

Plan apochromatic oil immersion objective with superior color correction, optimized for www.leica-microsystems.com 5 I 11

Items confocal scanning applications.

Free working distance: 0.24 mm.

Obj. HC PL APO 63x/1.40 OIL CS2

15506350

Quantity

Plan apochromat oil immersion objective with superior color correction, optimized for confocal scanning applications.

Free working distance: 0.14 mm.

I

Items

Air Table 750x900 w/Casters & OnTrak 29"

8114949

Quantity

Air Table 750mm X 900mm #63-44324-03 with Casters. Include package of 8112184 ,,TMC cleanbench table with 29" total floating height with casters and ontrack installation.

I Scan Head

STELLARIS 5 LIAchroic

158101101

Items Quantity

STELLARIS 5 LIAchroic is a true confocal point scanning system with LIAchroic beam splitters. It includes a highly sensitive, prism-based spectral detection design with computer controlled adjustable bandwidth for all fluorescence channels.

Up to 2 different laser ports are available, dependent on the configuration, for incoupling of

405 nm and visible lasers.

In combination with the new Power HyD S detectors STELLARS 5 LIAchroic offers

210 maximized detection efficiency for all commonly available fluorophores and allows the application and separation of multiple spectrally overlapping marker, up to five simultaneously.

The combination of Field-of-View (FOV) or Tandem scanner (optional) with Dynamic Signal

Enhancement and LIGHTNING delivers superb image quality at full speed and in super resolution.

STELLARIS Spectral Detection (SP)

• Up to five fully tunable spectral detection channels

• Maximal fluorescence detection because of unique prism design www.leica-microsystems.com 6 I 11

• Detection range 410-850 nm. Spectral tuning resolution 1 nm. Minimal detection range 5 nm

• Equal brightness between channels due to highly efficient W-shaped slit design

• permits excitation scans as well as unique excitation-emission ("lambda-square") scans for confocal spectroscopy applications

STELLARIS 5 LIAchroic laser supply unit

• Up to 6 single laser lines (405, 448, 488, 514, 561, 638 nm)

Contains a three-mirror scanner design for:

• Parallax-free imaging

• Maximal field-of-view

Supports two types of scan optics:

• HIVISR with scan rotation

• HIVISR

Supports extensions for:

• DLS - Digital Light Sheet Microscopy

Supports the fluorifier discs with numerous options for:

• Polarization selective imaging

• Optional notch filters for additional reflection suppression

Core software functionalities:

• Image Compass: Simplistic user interface, even for complex experiments. Set up of multicolor experiment with one click per fluorophore. Maximal signal yield through optimal acquisition settings selected automatically. Multidimensional acquisition, full control of motorized hardware

• Dynamic Signal Enhancement: Uncompromised temporal resolution up to 290 fps maintaining optimal signal to noise ratio

• LIGHTNING: LIGHTNING basic detection package. Fully automated online data acquisition with super-resolution down to 120 nm. Adaptive image quality determination and reconstruction

• Navigator: Navigator basic package. Spiral scan to easily generate an overview of your specimen. Stitching plus mark and find functionalities.

• Lambda scan: Acquisition of emission spectrum using spectral detectors. Acquisition of excitation spectrum using white light laser

• Lambda-lambda scan: Acquisition of full excitation-emission spectrum

• Z intensity compensation: Laser power and/or detector gain adjustments within z stacks

Other features are:

• Support for optional bright field detector

• Single square detection pinhole for maximal optical resolution

• Automatic and user-friendly optimization of the pinhole diameter, wavelength-dependent automatic mode available

• Internal scanner controlled via FPGA (Field programmable gate arrays) www.leica-microsystems.com 7 I 11

Items Quantity

• Multidimensional image acquisition in x, y, z (space dimension) and t (time), lambda

(wavelength) and any combination thereof

• Supports optional z-galvanometer or z-piezo stage for real-time cross-sections

• User friendly, intuitive LAS X control software

• ROI (region of interest) scan with arbitrary geometry independent for each laser line

• Sequential Scanning for cross-talk free multi-color imaging (stack- wise, frame- wise or line-wise)

• Beam parking for local fluorescence photobleaching, laser ablation or (optional) fluorescence correlation spectroscopy

• Programmable control panel for efficient real-time control of all relevant system parameters

LIAchroics RGB

158209433

LIAchroic beam splitter for STELLARIS 5 LIAchroic ( 158101101 ). The beam splitters have been custom designed and produced by Leica and thus provide excellent image contrast with optimal transmission up to 850 nm. The beam splitters have the following properties:

- Low incident angle for best transmission independent of polarization of fluorescence light

- Neutral beam splitter with splitting ratio of 15/85

- LIAchroic beam splitter for 638 / 561 / 488

- Support for laser combination Red/Green/Blue

System requirements:

- STELLARIS 5 LIAchroic ( 158101101)

FOV Scanner STELLARIS 5

158101130

Field-of-View scanning system for STELLARIS 5.

Highest axial resolution with galvanometric scanner: 5 confocal and fully multi-spectral channels along with transmission light detection, simultaneously. True optical sections by point scanning, compatible with AO BS® and SP® detection.

-True confocal point scanner for highest axial image resolution

-User adjustable speed 1 - 2600 Hz in steps of 1 Hz

-X2Y-Scanner design for homogeneous illumination in a largest field of view

-Feedback monitoring of the scanner for constant image quality

-Actively cooled scanner

-Switch between linearized and sinusoidal x-scan

-Linearized scanner movement with constant, very long pixel dwell time and short duty cycle (< 15%) to ensure for a steady illumination time per pixel

-Up to 10 frames / second , 512 x 512

-Up to 131 frames / second , 512 x 16

-Max. Image resolution 8192 x 8192 or 64 Mpixels

-Line frequency up to 5200 Lines / second

-Very large scanfield with 22 mm (FOV) diagonal in the intermediate image plane www.leica-microsystems.com s I 11

Items Quantity

-Hardware zoom, stepless 0.75x - 48x

-Optical scan field rotation: 200° (Koenig-Rotator), optional

Scan optics HIVISR with rotation DMi8

158101120

The scan optics module contains the complete scan optics for STELLARIS 5 on DMi8 stands with the following features:

-Optical coating HIVISR with high transmission of> 99% throughout the visibile spectrum ranging from 400-850 nm

-Low reflection at optical surfaces throughout the visible spectrum

- Independent optical rotation of the scanfield up to around 200° by a highly transparent

Abbe-Konig rotator design

-Prepared for either an FOV or a tandem scanner with Leica's parallax- free three-mirror design

-Prepared for excitation with 405 nm cw or pulsed laser, all cw lasers emitting visible light and white light laser

Power HyD S 2ch SP Core Unit

158101312

Power Hybrid Detector HyD S: The new standard for detection. All-round detector, high performance throughout the spectrum, two times increased photon detection efficiency in the blue-green range compared to standard multialkaline photomultiplier. Leica Si-based technology.

-Typical photon detection efficiency> 58% @ 500nm.

-Multi-pixel silicon photo-multiplier array.

-Low dark noise

-Extended dynamic range for maximal contrast and flexibility

-Analog, reflection and photon counting detection modes

-Power counting: 0, 1,2 (double) photons can be identified

-TauSense capabilities (requires a pulsed laser source)

-TauGating: Up to 2 tunable timegates, simultaneousely. TauContrast: Mean photon arrival time. TauSeparation: Lifetime based component separation.

Power HyD S SP 3th ch

158101313

Power Hybrid Detector HyD S: The new standard for detection. All-round detector, high performance throughout the spectrum, two times increased photon detection efficiency in

260 the blue-green range compared to standard multialkaline photomultiplier. Leica Si-based technology.

-Typical photon detection efficiency> 58% @ 500nm.

-Multi-pixel silicon photo-multiplier array.

-Low dark noise

-Extended dynamic range for maximal contrast and flexibility www.leica-microsystems.com s I 11

Items Quantity

-Analog, reflection and photon counting detection modes

-Power counting: 0, 1,2 (double) photons can be identified

-TauSense capabilities (requires a pulsed laser source)

-TauGating: Up to 2 tunable timegates, simultaneousely. TauContrast: Mean photon arrival time. TauSeparation: Lifetime based component separation.

Power HyD S SP 4th ch

158101314

Power Hybrid Detector HyD S: The new standard for detection. All-round detector, high performance throughout the spectrum, two times increased photon detection efficiency in the blue-green range compared to standard multialkaline photomultiplier. Leica Si-based technology.

-Typical photon detection efficiency> 58% @ 500nm.

-Multi-pixel silicon photo-multiplier array.

-Low dark noise

-Extended dynamic range for maximal contrast and flexibility

-Analog, reflection and photon counting detection modes

-Power counting: 0, 1,2 (double) photons can be identified

-TauSense capabilities (requires a pulsed laser source)

-TauGating: Up to 2 tunable timegates, simultaneousely. TauContrast: Mean photon arrival time. TauSeparation: Lifetime based component separation.

SP Light Trap

158201310

Housing for protection against reflections and dust from SP channels without detector. This position can be equipped with an additional confocal detector as part of an upgrade.

STELLARIS 5 DMi8 adapter

158101140

Mechanical part essential for STELLARIS 5 scan head on DMi8 stand

I Laser UV - VIS

# Items Quantity

Laser Port 405

158101200

Optical and mechanical coupling interface for laser with continuous wave 405 nm

-Laser coupling via fiber, beam merging by specific longpass-dichroic LP 425

-Unified collimation optics compatible with CS2 design HC PL APO objecive lenses

-Chromatic correction for optimal multi-color imaging

-Streamlined optical design for all magnifications for reduced maintanance cost www.leica-microsystems.com 10 I 17

-UV collimator automated by software

Simultaneous configuration of UV and VIS lasers is possible due to 2 independent laser ports in scan head.

Laser 405 DMOD

158202140

Laser Diode with wavelength 405 nm cw for fluorescence imaging with dyes such as DAPI or Hoechst. Laser intensity is directly modulated (DMOD), suitable for imaging with region of interest (ROI), in detail:

310 - Wavelength 405±3 nm

- Laser output power 50 mW

- Direct modulation of laser intensity (DMOD)

- Suitable for imaging and photoactivation

System requirements:

- Laser Port 405 nm

- CS2 objective lens for optimal multi color imaging

Laser 488nm (Blue)

15820211 2

Solid state laser "Blue" 488 nm cw Laser intensity is modulated via acousto-optical tunable filter (AOTF)

320 Specifications:

- Wavelength 488±1 nm

- 20 mW output power

- Laser class lllb

Laser 561 nm (Green)

158202114

DPSS laser "Green" 561 nm cw Laser intensity is modulated via acousto-optical tunable filter (AOTF)

330 Specifications:

- Wavelength 561 ±1 nm

- 20 mW output power

- Laser class lllb

Laser 638nm (Rot)

158202115 www.leica-microsystems.com

11 I 17

Laser diode "Red" 638 nm cw Laser intensity is modulated via acousto-optical tunable filter

(AOTF) Specifications:

- Wavelength 638 +2/-1 nm

- 30 mW output power

- Laser class lllb

I Software and Workstation

High brilliance monitor

158003150

Items

- Display size (diagonal): 95 cm (37,5 inch)

- Resolution (native): 4K-Wide 3840 x 1600 @ 60 Hz

- Contrast ratio: 1000:1 static, 5000000:1 dynamic

350 - Display: IPS with LED-Backlight

- Angle: 178° horizontal, 178° vertical

- Proportion: 21 :9

- Display range (metric): 87,97 x 36,65 cm

- Brightness: 300 cd/m2

Quantity

- Type of display input: 1 DisplayPort, 1 HDMI, 1 USB Type-C

- Point spacing: 0,229 mm

- Reaction time: 5 ms grey to grey

Workstation Premium

158203112

"High-end workstation HP Z6G4 with CUDA support for high workloads. Can be combined with all advanced imaging modalities (DIVE, DLS, FALCON, STED). Good performance of

Lightning, very good performance of Navigator and Rolling Average:

360 - Intel Xeon Gold 6244 processor (8 cores I 16 threads).

- 96 GB working memory.

- Nvidia Quadro RTX 5000 graphics card with 16 GB graphic card memory and 3072 CUDA cores.

- fast 256 GB SATA SSD system drive (Windows, LAS X), very fast 2 TB M.2 SSD hard drive for temporary storage of data (transient data container of LAS X, not for the user) and normal 6 TB HDD drive for storage of data.

- Intel X790 network adapter.

- HP 9.5mm Slim SuperMulti DVD writer.

www.leica-microsystems.com 12 I 17

- Windows 10 loT Enterprise LTSC 2019.

- Keyboard, mouse and mouse pad.

- 2 Thunderbolt ports (TB3).

The specifications of the PC may change due to technical innovations."

LAS X STELLARIS Control Software

158203200

"LAS X Stellaris Control Software':

- Leica LAS X system software for control of scan process and image processing.

- Control of motorized hardware.

- Multidimensional image acquisition.

- GUI optimized for dark rooms and image processing.

- Software-aided hardware configuration based on fluorophores used.

- Processing, quantification and easy export of data.

- Extension of functionality with additional licenses.

- Online software and hardware manual.

- Includes hardware dongle and installation CD.

- Mandatory for all confocal systems.

Includes 'LAS X Lightning' (without Lightning Expert) and 'LAS X Navigator'.

'LAS X Lightning' (not including Lightning Expert) Basic Leica detection software package for confocal, STED and multiphoton data. LAS X LIGHTNING provides access to the

LIGHTNING detection concept, for fully automated online data acquisition with the

370 following content:

- Super-resolution down to 120 nm in x and y, 200 nm in z.

- Wizard for online data acquisition.

- Fully automated "One-Click" functionality.

- Additional detection strategies for Low SNR & High SNR.

- Adaptive image quality determination and adaptive reconstruction in automatic mode.

- Elimination of boarder effects.

- 2D reconstruction.

- Time series.

- Channel-independent image reconstruction.

- High-performance GPU-based processing.

'LAS X Navigator' including the 'LAS X Stitching', 'LAS X Mark and Find' as well as the

'control software for the water immersion micro dispenser':

- A spiral scan can be used for a search in the vicinity of your current location.

- Overview images of any shape can be generated with a user defined region.

- Includes a choice of predefined sample carrier formats such as slides, petri dishes, chambered slides and well plates from different suppliers.

www.leica-microsystems.com 13 I 17

- Images can be added to the overview no matter which magnification, camera, detector or contrasting method is used.

- An unlimited number of regions and positions can be defined for high resolution scans and multiwell projects.

LAS X 3D Visualisation

158203202

"LAS X 3D Visualisation' is a software module for the reconstruction and processing of 3D data:

- Fast motion and processing by GPU-based algorithms.

- Various projection modes: Transparent, Maximum Intensity, and Depth Coding.

380 - Individual settings for intensity, minimum and maximum grey level, gamma and opacity for each channel.

- User defined interactive shadow projection to emphasize three dimensional structures.

- Auto motion function to emphasize the spatial effect of the 3D volume.

- Powerful clipping tool for channel specific user defined segmentations.

- Sophisticated Movie Editor for the generation of complex 3D animations.

- Orthogonal Sectioning tool, including rotatable section planes and 3D cropping capabilities.

- Annotation tool to add comments to a 3D volume that are saved with the data set.

- Stereo images (cyan / magenta, horizontal and vertical shutter, quad- based).

LAS X Dye Finder

158203206

"LAS X Dye Finder' is a software module for the separation of different fluorescence

390 signals:

- Automatic, channel and spectral mode.

- Online dye separation during image acquisition.

LAS X Offline Software

158203251

4oo 'LAS X Offline Software' runs on PCs with Windows 10 (64-bit) and contains licenses for:

LAS X Basic license, Live Data Mode, Colocalization, FRAP, Dye Finder, Electrophysiology.

Computer table small 80x80 cm

158004731

Computer table 80x80 cm (31.5 x 31.5 inch), surface off-white, legs: tubular, metal.

www.leica-microsystems.com 14117

I Service

Service Installation

9I_LL_STELLARIS_B

Training

8074150

Items www.leica-microsystems.com

Quantity

15 I 17 www.leica-microsystems.com 16 I 17

Science Lab

MICROSYSTEMS

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Leica Microsystems Inc

1700 Leider Lane

Buffalo Grove, IL 60089

Leica Biosystems and Leica Microsystems are part of Leica

Microsystems Inc.

www.leica-microsystems.com

LEARN I SHARE I CONTRIBUTE

www.leica-microsystems.com/science-lab

Phone:

800-526-0355

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847-236-3009

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