Statement of Work_multiomics_1.doc
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- Attached to
- RNA Sequencing on Chicken Muscle Samples Federal contract opportunity
- Solicitation number
- 12405B23Q0655
About this file
This statement of work outlines mRNA sequencing services required by the US Department of Agriculture Agricultural Research Service. The contractor will conduct mRNA sequencing and bioinformatics analysis on 50 chicken muscle samples provided by the government. Services include RNA sample quality control, mRNA library preparation using poly-A enrichment, Illumina sequencing with a minimum of 6 gigabytes of raw data per sample, and standard bioinformatics analyses. Required bioanalyses consist of novel gene prediction, alternative splicing analysis, single nucleotide polymorphism/insertion-deletion detection, gene expression quantification, differential expression analysis, gene ontology enrichment of differentially expressed genes, gene set enrichment analysis, KEGG and Reactome pathway enrichment, and protein-protein interaction analysis. The government will not provide any property for the work, which is solicited under opportunity 12405B23Q0655 to support agricultural research publications.
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Other files for this federal contract opportunity
| File | Type | Posted |
|---|---|---|
| Service Provisions 1109175.docx | DOCX document |
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Statement of Work (SOW) mRNA sequencing of chicken muscle samples for USDA-ARS, USNPRC, QUALITY & SAFETY ASSESSMENT RESEARCH UNIT
GENERAL INFORMATION
Scope of Work: This contract is located at the USNPRC at 950 College Station Road Athens GA, 30605. A company/analytical service will conduct mRNA sequencing and provide bioinformatics analysis of chicken muscle samples provided by ARS. The company/analytical service will perform all experiments at their location. The purpose of this SOW is to acquire data that will support publications in peer reviewed journals. Analyses and deliverables include:
Plant and animal eukaryotic mRNA-seq with reference (WBI)
- RNA sample QC
- mRNA library preparation (poly A enrichment)
- Illumina sequencing platform (e.g. NovaSeq, HiSeq, etc.)
- 6 G raw data per sample
- 50 samples
- Standard bioinformatics analyses including:
- Novel Gene Prediction
- Alternative Splicing Analysis
- SNP/InDel Analysis
- Gene Expression Quantification
- Differential Expression Analysis
- GO Enrichment Analysis of Differentially Expressed Genes (DEGs)
- GSEA Enrichment Analysis of Expressed Genes
- KEGG Pathway Enrichment Analysis
- Protein Protein Interaction Analysis
- Reactome Pathway Enrichment Analysis o Government Furnished: No Government-furnished property will be provided.
Contractor Furnished Items and Services: GENERAL. Contractor shall furnish everything required to perform this Statement of Work.
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